Yael Gernez, MD, MS
Post Doctoral Fellow
Training as a physician. Starting in 1998 at one of the top Medical School's (Lariboisiere University in Paris, France) I completed my program in the top 5% (249 of 5000 french medical students). Before starting my residency, in the summer of 2002, I spent three months as an intern in the Cardiology and Emergency Room at the Sacre Coeur Hospital in Montreal, Canada. As an Intern, I had a great opportunity to, not only live abroad and work in a hospital, but also to learn about how medicine is practiced in other countries. Then, I went to the University of Marseilles, where I completed my adult pulmonary residency. In 2007, I received the Gold Medal Award (first place) for medical interns. During my residency, I completed a Master of Sciences (2005-2006).
First experience in clinical Research: In 2005, working in the Herzenberg Laboratory for one year, I helped my primary mentor, Dr. Rabindra Tirouvanziam (Instructor in the department of Pediatrics at Stanford). Dr. Tirouvanziam developed a direct mutiparameter FACS method for neutrophil analysis in whole blood. My work was to help to develop and conduct a randomized, placebo-controlled, double-blind phase 2a trial of high-dose oral N-acetylcysteine in cystic fibrosis trial in collaboration with Dr. Moss and Dr. Conrad. The result of this one years work was the publication of two studies that appear in the PNAS, USA (March 2008) and the Journal of Allergy and Clinical Immunology thank to a collaboration with Dr. Nadeau (September 2007). I was respectively second and first author on these publications. Further, I co-wrote, with Dr. Tirouvanziam, a review article published in the main review for French Pulmonologists, the Revue des Maladies Respiratoires (2007). In 2006, I returned to France to finish my residency in adult pulmonary medicine.
First two years of postdoctoral work at Stanford. Just after I obtained my MD, I came back to Stanford as a post-doctoral fellow, I decide to focus my attention on granulocytes to explore the mechanism of atopic diseases in humans. Thanks to the Dean and Morgridge and Gallo Fellowships and in collaboration with Dr. Nadeau and Dr. Tirouvanziam, a pivotal step in our study was the successful development of a direct multiparameter FACS method for basophil and eosinophil analysis in whole blood. I developed assays to determine their phenotype as well as their intracellular signaling pathways from 50 microliters per assay of whole blood. I also mentored a current Medical Student at University California, San Diego, T. Nguyen. Her project was on Eosinophilic esophagitis, we submited her manuscript to « Journal of Pediatric Gastroenterology and Nutrition ». Futhermore, these FACS-based assays formed the basis of an in press article in Inter. Arch, of All. and Immun. journal. The basophil CD203c and CD63 assay can be developed into a valid and facile method for identifying offending allergens and monitoring responses to therapy. Last summer, I mentored a prospective medical student, T. Jauhari.
Current focus: Now, I am shifting focus to the issue of allergic bronchopulmonary aspergillosis (ABPA), a very intriguing and poorly understood chronic infection with an eukaryotic pathogen in patients with cystic fibrosis
Phosphorylated STAT1 and STAT6 in blood leukocytes as disease indicators in Eosinophilic Esophagitis. Y. Gernez, T. Nguyen, J. Fuentebella, A. Patel, R. Tirouvanziam, N. Reshamwala, V.E. Saper, D. Bass, W.E. Berquist, K.L. Cox, J.A. Kerner, K.C. Nadeau. American Journal of Gastroenterology, submitted
Basophil CD203c levels are increased at baseline and can be used to monitor omalizumab treatment in subjects with nut allergy. Y. Gernez, R. Tirouvanziam, G. Yu, E.E.B. Ghosn, N. Reshamwala, T. Nguyen, M. Tsai, S.J. Galli, L.A. Herzenberg, L.A. Herzenberg, K.C. Nadeau, International Archives of Allergy and Immunology, in Review
Neutrophils in chronic inflammatory airway diseases: can we target them and how. Y. Gernez, R. Tirouvanziam and P. Chanez. ERJ, 2010 accepted.
Activation of critical, host-induced, metabolic and stress pathways marks neutrophils entry into cystic fibrosis lungs. M. Makam, D. Diaz, J. Laval, Y. Gernez, C.K. Conrad, C.E. Dunn, Z.A. Davies, R.B. Moss, L.A. Herzenberg, L.A. Herzenberg and R. Tirouvanziam. Proc Natl Acad Sci U S A. 2009, 106:5779
Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways. R.Tirouvanziam, Y. Gernez, C.K. Conrad, R.B. Moss, I. Schrijver, C.E. Dunn, Z.A. Davies, L.A. Herzenberg, L.A. Herzenberg. Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4335-9. Epub 2008 Mar 11.
A rare cause of stridor in a 59 year old woman in intensive care. Y. Gernez, D. Breen, J.M. Peloni, B. Meric, H. Dutau. Revue des Maladies Respiratoires, 2008 Sep;25(7):871-4
Altered phosphorylated signal transducer and activator of transcription profile of CD4(+) CD161(+) T cells in asthma : modulation by allergic status and oral corticosteroids Y. Gernez, R. Tirouvanziam, K.D. Nguyen, L.A. Herzenberg, A.M. Krensky and K.C. Nadeau. J Allergy Clin Immunol. 2007 Dec;120(6):1441-8. Epub 2007 Oct 24.
- Highlighted in JACI 2008
Phospho Facs : a powerful tool for exploring intracellular transduction cascades. Y. Gernez, L.A. Herzenberg , L.A. Herzenberg, R. Tirouvanziam. Rev Mal Respir. 2007 Oct;24(8):955-64. Review. French.
A contributive result of open-lung biopsy improves survival in acute respiratory distress syndrome patients L. Papazian, C. Doddoli, B. Chetaille, Y. Gernez, C. Zandotti, X. Thirion, P. Thomas. Critical Care Medicine, 2007 Mars ;35(3) :755-62
Hypersensitivity to carboplatin. An effect of generic drugs? Y. Gernez, B. Barlesi, P. Astoul, A. Magnan. Revue des Maladies Respiratoires, 2006 Jun, 23(3) 269-272
Acute respiratory distress syndrome following inhalation of barium sulfate. Y. Gernez, F. Barlesi, B. Chetaille, J.M. Forel, L. Papazian, P. Astoul. Revue des Maladies Respiratoires, 2005 Jun, 22(3) 477-80
An integrative approach for immune monitoring of human health and disease by advanced flow cytometry methods. R. Tirouvanziam, D. Diaz, J.Laval, M. Makam and Y. Gernez
Gernez Y., K. Donis, N. Reshamwala, D.B. Lewis, K.C. Nadeau. Disseminated Nontuberculous Mycobacterial infection in a Vietnamese Woman. PID Immunology 2009
Y. Gernez, K. Donis, D.B. Lewis, K.C. Nadeau. A case of adult with PID. 2008: Clinical Immunology Society conference
Y. Gernez, R. Tirouvanziam, K.D. Nguyen, L.A. Herzenberg, A.M. Krensky and K.C Nadeau. Altered phosphorylated signal transducer and activator of transcription profile of CD4(+) CD161(+) T cells in asthma : modulation by allergic status and oral corticosteroids. 2007 : European Respiratory Conference, CPLF
Patent filed on November 9, 2009
Y. Gernez (inventor), Rabindra Tirouvanziam, Kari Nadeau and Leonore Herzenberg (2009): Methods and assays for detecting and quantifying pure subpopulation of white blood cells in immune system disorders. Stanford University Office of Technology Transfer, Stanford, CA
Patent Filed January, 2010
Y. Gernez (inventor), Rabindra Tirouvanziam, Kari Nadeau and Leonore Herzenberg (2009): Granulocyte-based methods for the detection and monitoring of immune system disorders.