LAH Publications
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LAH #001 Mitchell, H. K. and Herzenberg, L. A. (1955). Enzymatic degradation of cytohrome C. Methods of Enzymology. C. a. Kaplan. New York, Academic Press. 60: 167-169
LAH #002 Mitchell, H. K. and Herzenberg, L. A. (1957). “Zone electrophoresis on sponge rubber.” Anal. Chem 29(8): 1229.
LAH #003 Herzenberg, L. A., Herzenberg, L.A. (1959). “Adaption to Lactose.” Nutrition Reviews 17: 65-67.
LAH #004 Herzenberg, L. A. (1959). “Studies on the induction of b-galactosidase in a cryptic strain of escherichia coli.” Biochem. et Biophys. Acta 31: 525-538.
LAH #005 Herzenberg, L. A. (1960). Discussion of genetics of somatic cells. Methodology in Mammalian Genetics. San Francisco, Holden-Day, Inc.: 462-463.
Herzenberg, L. A., R.A. Roosa (1959). “Nutritional Requirements for growth of a mouse lymphona in cell culture.” Exp. Cell Res. 21: 430-438.
Herzenberg, L. A. (1961). Isolation and Identificatio of Derivatives Formed in the Course of Intracellular Accumulation of Thiogalactosides by Escherichia coli. Archives of Biochemistry and Biophysics. 93: 314-315
Cann, H. M., Herzenberg, L.A. (1961). “Detection and Localization of H-2 Antigen in Cells Grown in Culture.” Am. J. Diseases of Children 102(4): 477.
Herzenberg, L. A. (1962). Chemical and Serological Characterization of Purified H-2 Antigens. Mechanisms of Immunological Tolerance. Prague, Publishing House of the Czechoslovak Academy of Sciences: 495-499.
Ames, B., Garry, B. and Herzenberg, L. (1961). “Association of H-2 antigens with the cell membrane fraction of mouse liver.” Proc. Nat. Acad. Sci 47: 762-767.
Herzenberg, L. A., Herzenberg, L.A. (1961). “Association of H-2 Antigens with the Cell Membrane Fraction of Mouse Liver.” Proceedings of the National Academy of Sciences 47: 762-767.
Herzenberg, L. A., Gonzales, B. (1962). “Appearance of H-2 Agglutinins in Outcrossed Female Mice.” Proceedings of the National Academy of Sciences 48(4): 570-573.
LAH #012
Herzenberg, L. A. (1962). Histocompatibility Antigens Tissue Transplantation. American Conference of Physicians, Annual Meeting.
Herzenberg, L. A. (1962). A Genetic and Immunologic Approach to the Purification of an Histocompatibility Antigen. The Effects of Ionizing Radiations on Immune Processes. C. A. Leone. New York, Gordon and Breach Science Publishers: 365-379.
Ozer HL, H. L. (1962). “H-2 Immunogenicity of Liver Cell Membranes in the Mouse.” Transplantation Bulletin 30(1): 165/52-54.
Cann, H. M., Herzenberg, L.A. (1962). “In Vitro Selection for Isoantigenic Variants of Mammalian Somatic Cells.” Am. J. Diseases of Children: 533.
LAH #016
Herzenberg, L. A. (1962). “Steps Toward a Genetics of Somatic Cells in Culture.” J. of Cellular and Comparative Physiology 60(2): 145-151.
LAH #017
Herzenberg, L. A. (1962). “Maternal Isoimmunization as a Result of breeding in the Mouse.” J. of Cellular and Comparative Psyiology 60(2): 152-157.
Roosa, R. A., Bradley, T.R., Law, L.W., Herzenberg, L.A. (1962). “Characterization of Resistance to Amethopterin 8-Azaguanine and Several Fluorinated Pyrimidines in the Murine Lymphocytic Neoplasm, P388.” J. of Cellular and Comparative Physiology 60(2): 109-126.
Wunderlich, J. R., Herzenberg, L.A. (1962). “A second Gamma Globulin Isoatigen (allotype) in the Mouse.” Genetics 47: 995.
Cann, H. M., Herzenberg, L.A. (1963). “In Vitro Studies of Mammalian Somatic Cells.” The J. of Exp. Med. 117(2): 259-265.
LAH #020-01
Goodlin, R. and Herzenberg, L. (1964). “Pregnancy induced hemagglutinins to paternal H-2 antigens in the multiparous mice.” Transplantiation 2: 357-361.
LAH #020-02
Goodlin, R., Herzenberg, L. and De'Ath, S. (1964). “Isoimmunization associtaed with Ceserian section in the mouse.” Am. J. Obst. and Gynec. 90: 776-778.
Cann, H. M., Herzenberg, L.A. (1963). “In Vitro Studies of Mammalian Somatic Cell Variations. II” The J. of Exp. Med. 117(2): 267-284.
Mishell, R. I., Herzenberg, L.A., Herzenberg, L.A. (1962). “Leukocyte Agglutination in Mice: Detection of H-2 and non-H-2 Isoantigens.” The J. of Immun. 90(4): 628-633.
Herzenberg, L. A., Tachibana, D.K., Herzenberg, L.A. (1963). “A Gene Locus Concerned with Hemolytic Complement in Mus Musculus.” Genetics 48(5): 711-715.
Wunderlich, J., Herzenberg, L.A. (1963). “Genetics of a Gamma Globulin Isoantigen (Allotype) in the Mouse.” Proceedings of the National Academy of Sciences. 49(5): 592-598.
Herzenberg, L. A., Rosenberg, P., Herzenberg, L.A. (1963). “Gamma globulin isoantigens (allotypes) in the mouse.” Genetics 48: 892.
Herzenberg, L. A., Mishell, R.I., Herzenberg, L.A. (1963). “Gamma-globulin Isoantigens (Allotypes) in the House Mouse.” Proc. of the XI Intern. Congr. of Genetics, The Hague I: 196.
LAH #027
Warner, N. L., Herzenberg, L. A. and Goldstein, G. (1966). “Immunoglobulin isoantigens (allotypes) in the mouse. I. Genetics and Cross-Reaction of the 7S g2A-Isoatigens Controlled by Alleles at the Ig-1 Locus.” Journal of Experimental Medicine 121(3): 415-438.
Erickson, R. P., Herzenberg, L.A., Goor, R. (1964). “Partial Immune Elimination of Homologous Red Blood Cells in Mice.” Transplantation 2(2): 175-182.
Herzenberg, L. A., Cole, L.J. (1964). “Presence of Donor Specific Gamma-globulins in Sera of Allogeneic Mouse Radiation Chimeras.” Nature 202(4930): 352-353.
Goodlin, R. C. and Herzenberg, L. (1966). “Further studies on the mechanism of pregnancy-induced isoimmunization in mice.” American Journal of Obstetrics & Gynecology 95(1): 133-4.
Erickson, R. P., Tavhibana, D.K., Herzenberg, L.A., Rosenberg, L.T. (1964). “A Single Gene Controlling Hemolytic Compliment and a Serum Antigen in the Mouse.” J. of Immun. 92(4): 611-615.
Papermaster, B. W. and Herzenberg, L. A. (1966). “Isolation and characterization of an isoantigenic variant from a heterozygous mouse lymphoma in culture.” Journal of Cellular Physiology 67(3): 407-20.
Herzenberg, L. A. (1965). Antigen Variations of Samatic Cells and Cytogenic Aspects of Cell Antigenicity - Introduction. Genetic Variations in Somatic Cells - Proceedings of the Symposium on the mutational Process. Praha, Academia: 363-372.
Herzenberg, L. A., Herzenberg, L.A., (1965). Suppression of a gG-globulin allotype in mice by anti-allotype antibodies. Genetic Variations in Somatic Cells. Praha, Academia: 227-232.
Warner, N. L., Herzenberg, L. A. and Goldstein, G. (1966). “Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins.” Journal of Experimental Medicine 123(4): 707-21.
LAH #033
Warner, N. L. and Herzenberg, L. A. (1966). “Immunoglobulin isoantigens (allotypes) in the mouse. 3. Detection of allotypic antigens with heterologous antisera.” Journal of Immunology 97(4): 525-31.
Warner, N. L., Herzenberg, L. A., Cole, L. J. and Davis, W. E., Jr. (1965). “Dissociation of skin homograft tolerance and donor type gamma globulin synthesis in allogeneic mouse radiation chimaeras.” Nature 205(976): 1077-9.
Tyan, M. L., Cole, L. J. and Herzenberg, L. A. (1967). “Fetal liver: a source of immunoglobulin producing cells in the mouse.” Proceedings of the Society for Experimental Biology & Medicine 124(4): 1161-3.
Klein, J., Martinkova, J. and Herzenberg, L. A. (1967). “Analysis of the histocompatibility-2 (H-2) locus of NZB mice.” Transplantation 5(5): 1335-8.
Klein, J. and Herzenberg, L. A. (1967). “Congenic mouse strains with different immunoglobulin allotypes. I. Breeding scheme, histocompatibility tests, and kinetics of gamma G2a-globulin production by transferred cells for C3H.SW and its congenic partner CWB/5.” Transplantation 5(6): 1484-95.
Welton, J., Walker, S. R., Sharp, G. C., Herzenberg, L. A., Wistar, R., Jr. and Creger, W. P. (1968). “Macroglobulinemia with bone destruction.” American Journal of Medicine 44(2): 280-8.
LAH #039
Warner, N. L. and Herzenberg, L. A. (1967). “Immunoglobulin isoantigens (allotypes) in the mouse. IV. Allotypic specificities common to two distinct immunoglobulin classes.” Journal of Immunology 99(4): 675-8.
Minna, J. D., Iverson, G. M. and Herzenberg, L. A. (1967). “Identification of a gene locus for gamma-G-1 immunoglobulin H chains and its linkage to the H chain chromosome region in the mouse.” Proceedings of the National Academy of Sciences of the United States of America 58(1): 188-94.
Tyan, M. L., Cole, L. J. and Herzenberg, L. A. (1967). “Fetal liver cells: a source of specific immunoglobulin production in radiation chimeras. USNRDL-TR-67-26.” Research & Development Technical Report: 1-8.
Herzenberg, L. A., Goodlin, R. C. and Rivera, E. C. (1967). “Immunoglobulin synthesis in mice: suppression by anti-allotype antibody.” Journal of Experimental Medicine 126(4): 701-13.
LAH #043
Herzenberg, L. A., Minna, J.D., Herzenberg, L.A. (1967). “The Chromosome Region for Immunoglobulin Heavy Chains in the Mouse: Allelic Electrophoretic Mobility Differences and Allotype Suppression.” Cold SPring Harbor Symposia on Quantitative Biology XXXII: 181-186.
Herzenberg, L. A., H.O. McDevitt, L.A. Herzenberg (1968). “Genetics of Antibodies.” Annual Review of Genetics 2: 209-244.
Tyan, M. L. and Herzenberg, L. A. (1968). “Ontogeny of the mouse immune system: immunoglobulin producing cells. USNRDL-TR-68-57.” Research & Development Technical Report: 1-14.
LAH #045-01
Tyan, M. L. and Herzenberg, L. A. (1968). “Studies on the ontogeny of the mouse immune system. II. Immunoglobulin-producing cells.” Journal of Immunology 101(3): 446-50.
Tyan, M. L., McDevitt, H. O. and Herzenberg, L. A. (1969). “Genetic control of the antibody response to a synthetic polypeptide: transfer of response with spleen cells or lymphoid precursors.” Transplantation Proceedings 1(1): 548-5
Tyan, M., Herzenberg, L. and Cole), I. b. L. J. (1968). Immunoglobulin Production by Embryonic Tissues: Thymus Independent. Proceedings of the Society for Experimental Biology and Medicine. 12: 952-954.
Hulett, H. R., Bonner, W. A., Barrett, J. and Herzenberg, L. A. (1969). “Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence.” Science 166(906): 747-9.
Tyan, M. L., Herzenberg, L. A. and Gibbs, P. R. (1969). “Lymphoid precursors: thymus independent antibody production.” Journal of Immunology 103(6): 1283-7.
L'Age-Stehr, J. and Herzenberg, L. A. (1970). “Immunological memory in mice. I. Physical separation and partial characterization of memory cells for different immunoglobulin classes from each other and from antibody-producing cells.” Journal of Experimental Medicine 131(6): 1093-108.
Jacobson, E. B., L'Age-Stehr, J. and Herzenberg, L. A. (1970). “Immunological memory in mice. II. Cell interactions in the secondary immune response studies by means of immunoglobulin allotype markers.” Journal of Experimental Medicine 131(6): 1109-20,
Riblet, R. J. and Herzenberg, L. A. (1970). “Mouse lysozyme production by a monocytoma: isolation and comparison with other lysozymes.” Science 168(939): 1595-7.
Herzenberg, L. A. (1971). Gene Interaction in Immunglobulins. Human Anti-Human Gammaglobulins. G. a. Samuelsson. Oxford and New York, Pergamon Press: 3-15.
Chesebro, B. W., Mitchell, G. F., Grumet, F. C., Herzenberg, L. A. and McDevitt, H. O. (1970). Analysis of Cell Transfer Studies in a Genetic Control of the Immune Response in Mice. Cellular Interactions in the Immune Response. B. Karger. Buffalo, NY, Convoe Immunology: 83-92.
Herzenberg, L. A. (1970). “From cell biology to immunology--a short trip.” Journal of Cellular Physiology 76(3): 303-10.
Warner, N. L. and Herzenberg, L. A. (1970). “Tolerance and immunity to maternally derived incompatible IgG 2a -globulin in mice.” Journal of Experimental Medicine 132(3): 440-7.
Merrill, J. T., Veizades, N., Hulett, H. R., Wolf, P. L. and Herzenberg, L. A. (1971). “An improved cell volume analyzer.” Review of Scientific Instruments 42(8): 1157-63.
Mitchell, G. F., Chan, E. L., Noble, M. S., Weissman, I. L., Mishell, R. I. and Herzenberg, L. A. (1972). “Immunological memory in mice. 3. Memory to heterologous erythrocytes in both T cell and B cell populations and requirement for T cells in expression of B cell memory. Evidence using immunoglobulin allotype and mouse alloantigen theta markers with congenic mice.” Journal of Experimental Medicine 135(2): 165-84.
LAH #057-05
Chesebro, B. W., Mitchell, G. F., Grumet, F. C., Herzenberg, L. A., McDevitt, H. O. and Wegmann, T. G. (1972). “Cell transfer studies in a genetically controlled immune response.” European Journal of Immunology 2(3): 243-8
Mitchell, G., Mishell, R. and Herzenberg, L. (1971). Studies on the influence of T cells in antibody production. Progress in Immunology. New York, Academic Press: 323-335.
Herzenberg, L. A., R.G. Mage (1971). Allotypes. II. Presented at the first International Congress of Immunology. Progress in Immunology. New York, Academic Press: 1249-1251.
Herzenberg, L. A. (1971). “Immunoglobulin genetics in cellular immunology.” Annals of the New York Academy of Sciences 190: 130-5.
Herzenberg, L. A., Jacobson, E. B. and Riblet, R. J. (1971). “Chronic allotype suppression in mice: an active regulatory process.” Annals of the New York Academy of Sciences 190: 212-20.
LAH #062
Herzenberg, L. A. (1971). Discussion of separation of cells. Immunologic Intervention: II selective depletion of lymphocytes. New York: 96-104,145-148,281,289.
Jacobson, E. B. and Herzenberg, L. A. (1972). “Active suppression of immunoglobulin allotype synthesis. I. Chronic suppression after perinatal exposure to maternal antibody to paternal allotype in (SJL x BALB-c)F 1 mice.” Journal of Experimental Medicine 135(5): 1151-62.
Bonner, W. A., Hulett, H. R., Sweet, R. G. and Herzenberg, L. A. (1972). “Fluorescence activated cell sorting.” Review of Scientific Instruments 43(3): 404-9.
Herzenberg, L. A., L.A. Herzenberg (1973). Mouse immunolglobulin allotypes: Description and special methodology. Handbook of Experimental Immunology 2nd Edition. Oxford, Blackwell Scientific Publications: 13.1-18.
Jacobson, E. B., Herzenberg, L. A. and Riblet, R. (1972). “Active suppression of immunoglobulin allotype synthesis. II. Transfer of suppressing factor with spleen cells.” Journal of Experimental Medicine 135(5): 1163-76.
Julius, M. H., Masuda, T. and Herzenberg, L. A. (1972). “Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter.” Proceedings of the National Academy of Sciences of the United States of America 69(7): 1934-8.
Leute, R. K., Ullman, E. F., Goldstein, A. and Herzenberg, L. A. (1972). “Spin immunoassay technique for determination of morphine.” Nature - New Biology 236(64): 93-4.
Freed, J. H., Bechtol, K. B., Herzenberg, L. A. and McDevitt, H. O. (1973). “Analysis of anti-(T,G)-A--L antibody in tetraparental mice.” Transplantation Proceedings 5(1): 167-71.
Sarkar, S., Hyman, R., Masuda, T. and Herzenberg, L. A. (1973). “A rabbit antiserum to a thymus extract specific for mouse thymus-derived cells.” Journal of Immunology 110(5): 1222-32.
Jones, P. P., Cebra, J. J. and Herzenberg, L. A. (1973). “Immunoglobulin (Ig) allotype markers on rabbit lymphocytes: separation of cells bearing different allotypes and demonstration of the binding of Ig to lymphoid cell membranes.” Journal of Immunology 111(5): 1334-48.
Jan, W. H. and Herzenberg, L. A. (1973). Fetal erythrocytes detected and separated from maternal blood by an electronic fluorescent cell sorter. SAMA-UTMB, National Student research Forum, Galveston, TX.
Hulett, H. R., Bonner, W. A., Sweet, R. G. and Herzenberg, L. A. (1973). “Development and application of a rapid cell sorter.” Clinical Chemistry 19(8): 813-6.
Bobrove, A. M., Strober, S., Herzenberg, L. A. and DePamphilis, J. D. (1974). “Identification and quantitation of thymus-derived lymphocytes in human peripheral blood.” Journal of Immunology 112(2): 520-7
Herzenberg, L. A. (1973). “Curing Cancer by federal fiat.” Hospital Practice 8: 16
Freed, J. H. and Herzenberg, L. A. (1975). “Quantitative separation of antigen-specific murine antibodies by anti-allotype chromatography.” Scandinavian Journal of Immunology 4(5-6): 479-85.Abstract
Jones, P. P., Tacier-Eugster, H. and Herzenberg, L. A. (1974). “Lymphocyte commitment to Ig allotype and class.” Annales d Immunologie 125C(1-2): 271-6.
Herzenberg, L. A. and C. M. Metzler, Eds. (1974). Antibody induced suppressor T cells. The Immune System, Genes, Receptors, Signals. London, Academic Press, Inc.
Herzenberg, L. A. and Jones, P. P. (1974). “Mouse immunoglobulin allotypes: characterization and use in cellular immunology.” Annales d Immunologie 125C(1-2): 71-83.
LAH #076-01
Herzenberg, L. A. and Okumura, K. (1975). “Mechanism of allotype suppression. pp. 93-104.” In: Singhal SK, Sinclair NR, ed. Suppressor cells in immunity. London, Univ of Western Ontario.
Herzenberg, L. A., Chan, E. L., Ravitch, M. M. and Riblet, R. J. (1973). “Active suppression of immunoglobulin allotype synthesis. 3. Identification of T cells as responsible for suppression by cells from spleen, thymus, lymph node, and bone marrow.” Journal of Experimental Medicine 137(6): 1311-24.
Goodman, J. R., Julius, M. H. and Herzenberg, L. A. (1973). Ultrastructural visualization of KLH antigen on selected mouse spleen lymphocytes. 2nd International Symposium on Electron Microscopy and Cytochemistry., Amsterdam, North-Holland Publishing,Co.
Julius, M. H., Sweet, R. G., Fathman, C. G. and Herzenberg, L. A. (1975). “Fluorescence-activated cell sorting and its applications. pp. 107-21.” In: Richmond CR et al., ed. Mammalian cells: probes and problems. Oak Ridge, Tenn., Technical Information Center.
Julius, M. H., Simpson, E. and Herzenberg, L. A. (1973). “A rapid method for the isolation of functional thymus-derived murine lymphocytes.” European Journal of Immunology 3(10): 645-9.
Kreth, H. W. and Herzenberg, L. A. (1974). “Fluorescence-activated cell sorting of human T and B lymphocytes. I. Direct evidence that lymphocytes with a high density of membrane-bound immunoglobulin are precursors of plasmacytes.” Cellular Immunology 12(3): 396-406.
Epstein, L. B., Kreth, H. W. and Herzenberg, L. A. (1974). “Fluorescence-activated cell sorting of human T and B lymphocytes. II. Identification of the cell type responsible for interferon production and cell proliferation in response to mitogens.” Cellular Immunology 12(3): 407-21.
Jones, P. P., Cebra, J. J. and Herzenberg, L. A. (1974). “Restriction of gene expression in B lymphocytes and their progeny. I. Commitment to immunoglobulin allotype.” Journal of Experimental Medicine 139(3): 581-99.
Bechtol, K. B., Wegmann, T. G., Freed, J. H., Grumet, F. C., Chesebro, B. W., Herzenberg, L. A. and McDevitt, H. O. (1974). “Genetic control of the immune response to (T,G)-A--L in C3H in equilibrium C57 tetraparental mice.” Cellular Immunology 13(2): 264-77.
Jones, P. P., Craig, S. W., Cebra, J. J. and Herzenberg, L. A. (1974). “Restriction of gene expression in B lymphocytes and their progeny. II. Commitment to immunoglobulin heavy chain isotype.” Journal of Experimental Medicine 140(2): 452-69.
Herzenberg, L. A. (1974). “Short-term and chronic allotype suppression in mice.” Contemporary Topics in Immunobiology 3: 41-75.
Cantor, H., Simpson, E., Sato, V. L., Fathman, C. G. and Herzenberg, L. A. (1975). “Characterization of subpopulations of T lymphocytes. I. Separation and functional studies of peripheral T-cells binding different amounts of fluorescent anti-Thy 1.2 (theta) antibody using a fluorescence-activated cell sorter (FACS).” Cellular Immunology 15(1): 180-96.
Julius, M. H. and Herzenberg, L. A. (1974). “Isolation of antigen-binding cells from unprimed mice: demonstration of antibody-forming cell precursor activity and correlation between precursor and secreted antibody avidities.” Journal of Experimental Medicine 140(4): 904-20.
Fathman, C. G., Small, M., Herzenberg, L. A. and Weissman, I. L. (1975). “Thymus cell maturation. II. Differentiation of three "mature" subclasses in vivo.” Cellular Immunology 15(1): 109-28.
Herzenberg, L. A., C.M. Metzler, L.A. Herzenberg (1974). Mechanism of allotype suppression in mice. Immunological Tolerance: Mechnaisms and Potential Therapeutic Applications. New York, Academic Press: 519-529.
Loken, M. R. and Herzenberg, L. A. (1975). “Analysis of cell populations with a fluorescence-activated cell sorter.” Annals of the New York Academy of Sciences 254: 163-171.
Herzenberg, L. A. (1974). “Allotype suppression and production(?) by thymocytes and thymus-derived cells. pp. 111-8.” In: Brent L, Holborow J, ed. Progress in immunology II. Vol 2: Biological aspects I. Amsterdam, North-Holland.
Herzenberg, L. A. (1974). Chairman's Introduction, Symposium G 2nd International Congress on Immunology, Brighton, England, July 22-26, 1994. Progress in Immunology. Amsterdam, North holland American Elseveir. 2: 109.
Bechtol, K. B., Freed, J. H., Herzenberg, L. A. and McDevitt, H. O. (1974). “Genetic control of the antibody response to poly-L(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys in C3H--CWB tetraparental mice.” Journal of Experimental Medicine 140(6): 1660-75.
Herzenberg, L. A., Okumura, K. and Metzler, C. M. (1975). “Regulation of immunoglobulin and antibody production by allotype suppressor T cells in mice.” Transplantation Reviews 27: 57-83.
Weissman, I. L., Small, M., Fathman, C. G. and Herzenberg, L. A. (1975). “Differentiation of thymus cells-1,2.” Federation Proceedings 34(2): 141-4.
Herzenberg, L. A. and Sweet, R. G. (1976). “Fluorescence-activated cell sorting.” Scientific American 234(3): 108-17.
Stout, R. D., Yutoku, M., Grossberg, A., Pressman, D. and Herzenberg, L. A. (1975). “A surface membrane determinant shared by subpopulations of thymocytes and B lymphocytes.” Journal of Immunology 115(2): 508-12. ABSTRACT
Herzenberg, L. A., C.M. Metzler (1974). Antibody induced suppressor T-cells. The Immune System, Genes, Receptors, Signals. W. Sercarz, Fox. New York, Academis Press: 455-469.
Okumura, K., Metzler, C. M., Tsu, T. T. and Herzenberg, L. A. (1976). “Two stages of B-cell memory development with different T-cell requirements.” Journal of Experimental Medicine 144(2): 345-57. ABSTRACT
LAH #100
Herzenberg, L. A., Okumura, K. and Herzenberg, L. A. (1975). Mechanism of Allotype Suppression. Symposium on Suppressor Cells in Immunity, London, Ontario, Canada, University of Western Ontario.
Stout, R. D. and Herzenberg, L. A. (1975). “The Fc receptor on thymus-derived lymphocytes. I. Detection of a subpopulation of murine T lymphocytes bearing the Fc receptor.” Journal of Experimental Medicine 142(3): 611-21. Abstract
Functional studies of lymphoid cells defined and isolated by a fluorescent activated cell sorter (FACS) from normal abd athymic cells.1976Stout, R., S. D. Waksal, V. L. Sato, K. Okumura and L. A. Herzenberg (1976).Abstract
Julius, M. H., Janeway, C. A., Jr. and Herzenberg, L. A. (1976). “Isolation of antigen-binding cells from unprimed mice. II. Evidence for monospecificity of antigen-binding cells.” European Journal of Immunology 6(4): 288-92.Abstract
Stout, R. D. and Herzenberg, L. A. (1975). “The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor.” Journal of Experimental Medicine 142(5): 1041-51.Abstract
Loken, M. R., Sweet, R. G. and Herzenberg, L. A. (1976). “Cell discrimination by multiangle light scattering.” Journal of Histochemistry & Cytochemistry 24(1): 284-91.Abstract
Stout, R. D., Waksal, S. D. and Herzenberg, L. A. (1976). “The Fc receptor on thymus-derived lymphocytes. III. Mixed lymphocyte reactivity and cell-mediated lympholytic activity of Fc- and Fc+ T lymphocytes.” Journal of Experimental Medicine 144(1): 54-68.Abstract
Sato, V. L., Waksal, S. D. and Herzenberg, L. A. (1976). “Identification and separation of pre T-cells from nu/nu mice: differentiation by preculture with thymic reticuloepithelial cells.” Cellular Immunology 24(1): 173-85.
Petranyi, G. G., Keissling, R., Povey, S., Klein, G., Herzenberg, L. A. and Wigsell, H. (1976). “The genetic control of natural killer cell activity and its association with in viro resistance against a moloney lymphoma isograft.” Immunogenetics 3: 15-28.
Yutoku, M., Grossberg, A. L., Stout, R., Herzenberg, L. A. and Pressman, D. (1976). “Further studies on Th-B, a cell surface antigenic determinant present on mouse B cells, plasma cells and immature thymocytes.” Cellular Immunology 23(1): 140-57.
Okumura, K., Julius, M. H., Tsu, T. and Herzenberg, L. A. (1976). “Demonstration that IgG memory is carried by IgG-bearing cells.” European Journal of Immunology 6(7): 467-72.Abstract
Okumura, K., Herzenberg, L. A., Murphy, D. B. and McDevitt, H. O. (1976). “Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes.” Journal of Experimental Medicine 144(3): 685-98.Abstract
Herzenberg, L. A., Okumura, K., Cantor, H., Sato, V. L., Shen, F. W. and Boyse, E. A. (1976). “T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells.” Journal of Experimental Medicine 144(2): 330-44.Abstract
Murphy, D. B., Herzenberg, L. A., Okumura, K. and McDevitt, H. O. (1976). “A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes.” Journal of Experimental Medicine 144(3): 699-712.Abstract
Cremer, N. E., Herzenberg, L. A. and McDevitt, H. O. (1976). “Fifteenth midwinter conference of immunologists: lymphocyte differentiation.” Clinical Immunology & Immunopathology 6(3): 431-42.
Herzenberg, L. A., Black, S. J., Loken, M. R., Okumura, K., van der Loo, W., Osborne, B. A., Hewgill, D., Goding, J. W., Gutman, G. and Warner, N. L. (1977). “Surface markers and functional relationships of cells involved in murine B-lymphocyte differentiation.” Cold Spring Harbor Symposia on Quantitative Biology 41 Pt 1: 33-45.
Murphy, D. B., Okumura, K., Herzenberg, L. A. and McDevitt, H. O. (1977). “Selective expression of separate I-region loci in functionally different lymphocyte subpopulations.” Cold Spring Harbor Symposia on Quantitative Biology 41 Pt 2: 497-504.Abstract
Stout, R. D., Murphy, D. B., McDevitt, H. O. and Herzenberg, L. A. (1977). “The Fc receptor on thymus-derived lymphocytes. IV. Inhibition of binding of antigen-antibody complexes to Fc receptor-positive T cells by anti-Ia sera.” Journal of Experimental Medicine 145(1): 187-203.Abstract
Schroder, J. P. and Herzenberg, L. A. (1980). Prenatal diagnosis by cell sorting using a fluorescence-activated cell sorter(FACS). Genetic Disorders of the Fetus: Diagnosis, Precention and Treatment. New York, Plenum Press: 541-555.
Warner, N. L., Goding, J. W., Gutman, G. A., Warr, G. W., Herzenberg, L. A., Osborne, B. A., van der Loo, W., Black, S. J. and Loken, M. R. (1977). “Allotypes of mouse IgM immunoglobulin.” Nature 265(5593): 447-9.
Loken, M. R., Stour, R. D. and Herzenberg, L. A. (1979). Lymphoid cell analysis and sorting. Flow Cytometry and Sorting. M. Melamed, Mendelshon. New York, John H Wiley and Sons: 505-527
Loken, M. R., Parks, D. R. and Herzenberg, L. A. (1977). “Identification of cell asymmetry and orientation by light scattering.” Journal of Histochemistry & Cytochemistry 25(7): 790-5.Abstract
Herzenberg, L. A., V.T. Oi (1977). “A Rapid and Efficient Method for Preparing Purified Radio-labeled antibody: Use in a Solid-phase Radioimmune assay.”
Herzenberg, L. A., L.A. Herzenberg (1978). Mouse immunoglobulin allotypes: Description and specific methodology. Handbook of Experimental Immunology. D. Weir. Oxford, England, Blackwell Scientific Publications: 12.1-23.
Loken, M. R., Parks, D. R. and Herzenberg, L. A. (1977). “Two-color immunofluorescence using a fluorescence-activated cell sorter.” Journal of Histochemistry & Cytochemistry 25(7): 899-907.Abstract"] A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between
Goldsby, R. A., Osborne, B. A., Simpson, E. and Herzenberg, L. A. (1977). “Hybrid cell lines with T-cell characteristics.” Nature 267(5613): 707-8.
Golsby, R. A., Osborne, B. A., Simpson, E. and Herzenberg, L. A. (1977). Somatic Cell Hybrids with Tcell Characteristics. The Immune System: Genetics and Regulation. New Yokr, Academic Press: 265-271.
Herzenberg, L. A., L.A. Herzenberg (1976). Analysis and separation using the fluorescence activated cell sorter (FACS). Handbook of Experimantal Immunology. Oxford, England: 22.1-21
Black, S. J., van der Loo, W., Loken, M. R. and Herzenberg, L. A. (1978). “Expression of IgD by murine lymphocytes. Loss of surface IgD indicates maturation of memory B cells.” Journal of Experimental Medicine 147(4): 984-96.[showhide id="318129" link="Abstract"]B lymphocytes capable of generating primary IgM and IgG plaque-forming cells (PFC) responses to burro erythrocytes have surface IgD, as do primary IgM PFC. IgG memroy cells arising after one injection of antigen are divided into two groups, one of which expresses surface IgD while the other has no detectable membrane IgD. PFC generated from the IgG memory cells lacking surface IgD show a higher average avidity than those arising from IgD-positive IgG memory cells, indicating that mature IgG memory cells do not have surface IgD. After more than one injection of antigen, few, if any, IgG memory cells have surface IgD. IgG PFC arising in primary or secondary immune response lack membrane-bound IgD. These data provide the outlines for a B-cell maturation pathway in which IgD marks unprimed and early memory B cells and is lost in mature memory cells. Studies presented here were conducted by isolating IgD+ and IgD- cells with the fluorescence-activated cell sorter and functional testing of the isolated populations in adoptive transfer experiments.[/showhide
Black, S. J., Tokushia, T. and Herzenberg, L. A. (1980). “Memory B cells at successive stages of differentiation: expression of surface IgD and capacity for self renewal.” European Journal of Immunology 10(11): 846-51.[showhide id="318129" link="Abstract
Black, S. J., Goding, J. W., Gutman, G. A., Herzenberg, L. A., Loken, B. A., Osborne, W. and Warner, N. L. (1978). “Immunoglobulin isoantigen (allotypes) in the mouse V. Characterization of IgM allotypes.” Characterization of IgM allotypes.Immunogenetics 7: 213-230
Herzenberg, L. A., L.A. Herzenberg, C. Milstein (1978). Cell hybrids of myelomas with antibody forming cells and T-lymphomas with T cells. Handbook of Experimental Immunology, 3rd Edition. Weir. Oxford, England, Blackwell Scientific Publications: 25.1-7.
Nahm, M. H., Herzenberg, L. A., Little, K. and Little, J. R. (1977). “A new method of applying the Sips equation.” Journal of Immunology 119(1): 301-5.Abstract
Stout, R. D., Murphy, D. B., McDevitt, H. and Herzenberg, L. A. (1978). Ia antigens on FcR positive T Lymphocytes. Ir Genes and Ia Antigens. McDevitt. New York: 195-200.
LAH #134
Milstein, C. and Herzenberg, L. A. (1977). T and B Cell Hybrids. Regulatory Genetics of the Immune System: ICN-UCLA Symposia on Molecular and Cellular Biology, Los Angeles, CA, Academic Press.
van der Loo, W., Gronowicz, E. S., Strober, S. and Herzenberg, L. A. (1979). “Cell differentiation in the presence of cytochalasin B: studies on the "switch" to IgG secretion after polyclonal B cell activation.” Journal of Immunology 122(4): 1203-8.Abstract
Herzenberg, L. A., L. Wofsy (1977). Cell separation and characterization. mImmune System: Genetics and Regulation. New York, Academic Press: 341-353.
Stovel, R. T., Sweet, R. G. and Herzenberg, L. A. (1978). “A means for orienting flat cells in flow systems.” Biophysical Journal 23(1): 1-5.Abstract
Herzenberg, L. A., Bianchi, D. W., Schroder, J., Cann, H. M. and Iverson, G. M. (1979). “Fetal cells in the blood of pregnant women: detection and enrichment by fluorescence-activated cell sorting.” Proceedings of the National Academy of Sciences of the United States of America 76(3): 1453-5.Abstract
Oi, V. T., Jones, P. P., Goding, J. W. and Herzenberg, L. A. (1978). “Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens.” Current Topics in Microbiology & Immunology 81: 115-20.
Iverson, G. M., Goldsby, R. A. and Herzenberg, L. A. (1978). “Expression of Thy 1.2 antigen on hybrids of B cells and a T lymphoma.” Current Topics in Microbiology & Immunology 81: 192-4.
Osborne, B. A., Goldsby, R. A. and Herzenberg, L. A. (1978). “Selective expression of loci in th I--J region on T cell hybrids.” Current Topics in Microbiology & Immunology 81: 217-20.
Herzenberg, L. A. (1978). “The fluorescence-activated cell sorter (FACS): a retrospective and prospective view. pp. 99-109.” In: Knapp W, et al., ed. Immunofluorescence and related staining techniques. Amsterdam, Elsevier/North-Holland.
LAH #142-01
Tsu, T. and Herzenberg, L. A. (1979). Solid-Phase Radiioimmune Assays. Additional Methods: 373-397.
Goding, J. W., Oi, V. T., Jones, P. P. and Herzenberg, L. A. (1979). Monoclonal antibodies to alloantigens and to immunoglobulin allotypes. pp. 309-31. In: Pernis B, Vogel HJ, ed. Cells of immunoglobulin synthesis. New York, Academic Press.
Herzenberg, L. A. (1979). “Seek well and ye shall find [editorial].” New England Journal of Medicine 300(25): 1435-6
LAH #144
Oi, V., T. and Herzenberg, L. A. (1979). Hybrid Cell Lines. Selected Methods in Cellular Immunology. B. B. Mishell, W. H. Shiigi and W. H. Freeman. San Francsisco, W.H. Freeman Publishing: 17.1-17.11.
Kontiainen, S., Simpson, E., Bohrer, E., Beverley, P. C., Herzenberg, L. A., Fitzpatrick, W. C., Vogt, P., Torano, A., McKenzie, I. F. and Feldmann, M. (1978). “T-cell lines producing antigen-specific suppressor factor.” Nature 274(5670): 477-80.
Goldsby, R. A., Osborne, B. A., Suri, D., Mandel, A., Williams, J., Gronowicz, E. and Herzenberg, L. A. (1978). “Production of specific antibody without specific immunization.” Current Topics in Microbiology & Immunology 81: 149-51.
Simpson, E., Kontiainen, S., Herzenberg, L. A., Bohrer, E., Torano, A., Vogt, P., Beverley, P., Fitzpatrick, W. and Feldmann, M. (1978). “T cell hybrids with T cell functions.” Current Topics in Microbiology & Immunology 81: 195-202.
Goding, J. W., Black, S. J. and Herzenberg, L. A. (1978). Biosynthesis and two-dimensional gel analysis of IgM and IgD receptors. B Lymphocytes in the Immun Response. New York, Elsevier North-Holland: 9-13.
Black, S. J. Takeshi Tokuhisu and Herzenberg, L. A. (1979). “Regulation of B Cell Differentiation.” B Lymphocytes in the Immune Response Eds: Cooper/Mosier/Scher/Vietta, Elvesierf, North Holland. 147-9.
Black, S. J. and Herzenberg, L. A. (1979). “B-cell influences on the induction of allotype suppressor T cells.” Journal of Experimental Medicine 150(1): 174-83.
Hance, A. J., Robin, E. D., Simon, L. M., Alexander, S., Herzenberg, L. A. and Theodore, J. (1980). “Regulation of glycolytic enzyme activity during chronic hypoxia by changes in rate-limiting enzyme content. Use of monoclonal antibodies to quantitate changes in pyruvate kinase content.” Journal of Clinical Investigation 66(6): 1258-64.Abstract
Herzenberg, L. A., A.P. Dowsett, S.J. Black, L.A. Herzenberg (1979). Genetic and serologic requirements for indiction of spontaneous and anti-Ig induced allotype suppression,. B Lymphocytes in the immune response. New York, Elsvier North-Holland: 315-322.
Parks, D. R., Bryan, V. M., Oi, V. T. and Herzenberg, L. A. (1979). “Antigen-specific identification and cloning of hybridomas with a fluorescence-activated cell sorter.” Proceedings of the National Academy of Sciences of the United States of America 76(4): 1962-6.Abstract
Ledbetter, J., Goding JW, Tsu TT, Herzenberg LA (1979). “A new mouselymphoid alloantigen (Lgp100) recognized by a monoclonal rat antibody.” Immunogenetics 8: 347-360.
Herzenberg, L. A. and Black, S. J. (1980). “Regulatory circuits and antibody responses.” European Journal of Immunology 10(1): 1-11
Herzenberg, L. A., S.J. Black, T. Tokuhisa, L.A. Herzenberg (1979). Regulatory circuits and affinity maturation. Proceedings Columbia University College of Physicians and Surgeons Biomedical Symp., June 8-10, 1979. Regulatory T Lymphocytes. P. a. Vogel. New York, Academic Press: 417-439.
Oi, V. T. and Herzenberg, L. A. (1979). “Localization of murine Ig-1b and Ig-1a (IgG 2a) allotypic determinants detected with monoclonal antibodies.” Molecular Immunology 16(12): 1005-17.
Ledbetter, J. A. and Herzenberg, L. A. (1979). “Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.” Immunological Reviews 47: 63-90.
Herzenberg, L. A., J.A. Ledbetter (1979). Monoclonal antibodies and the fluorescence-activated cell sorter: Complementary tools in lymphoid cell biology. Molecular Basis of Immune Cell Function. Amsterdam, Elsevier/North-Holland Biomedical Press: 315-330.
Ledbetter, J., Goding JW, Tokuhisa T, Herzenberg LA (1980). Murine T cell differentiation and antigens detected by monoclonal antibodies. Monoclonal Antibodies. New York, Plenum Publishing Corp: 235-249.
Herzenberg, L. A., S.J. Black, L.A. Herzenberg (1980). Regulation of antibody responses. Strategies of Immune Regulation. New York: 157-177.
LAH #158a
Herzenberg, L. A., S.J. Black, T. Tokuhisa, L.A. Herzenberg (1980). A role of IgD: Dissociation of memory induction from affinity maturation. Strategies of Immune Regulation. New Yokr, Academic Press: 439-441.
Circuits for regulation antibody responses.1979Herzenberg, L. A., Sam J. Black , Leonard A. Herzenberg (1979). Circuits for regulation antibody responses. Molecular Basis of Immune Cell Function, Elseveir/North-Holland Biomedicaln Press: 147-160.
Micklem, H., S., Ledbetter, J. A., Eckhardt, L. A. and Herzenberg, L. A. (1980). Analysis of lymphocyte subpopulations with monoclonal antibodies to thy-1, lyt-1 and ThB antigens. Regulatory T Lymphocytes. New York, Academic Press: 119-13
Tokuhisa T, O. V., Gadus FT, Herzenberg LA. (1980). Induction of allotype suppression with monoclonal antibodies. Regulatory T Lymphocytes. New York, Academic Press: 315-328.
Black, S. J., T. Tokushia and L. A. Herzenberg (1980). "Memory B cells at successive stages of differentiation: expression of surface IgD and capacity for self renewal." European Journal of Immunology 10(11): 846-851.Abstract
Herzenberg, L. A., Black, S. J. and Tokuhisa, T. (1980). “Memory B cells at successive stages of differentiation. Affinity maturation and the role of IgD receptors.” Journal of Experimental Medicine 151(5): 1071-87.
Goding, J. W. and Herzenberg, L. A. (1980). “Biosynthesis of lymphocyte surface IgD in the mouse.” Journal of Immunology 124(6): 2540-7.Abstract
Herzenberg, L. A. and Tokuhisa, T. (1980). “Carrier-priming leads to hapten-specific suppression.” Nature 285(5767): 664-7.
Oi, V. T., Bryan, V. M. and Herzenberg, L. A. (1980). “Lymphocyte membrane IgG and secreted IgG are structurally and allotypically distinct.” Journal of Experimental Medicine 151(5): 1260-74.Abstract
Ledbetter, J. A., Evans, R. L., Lipinski, M., Cunningham-Rundles, C., Good, R. A. and Herzenberg, L. A. (1981). “Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man.” Journal of Experimental Medicine 153(2): 310-23.
Eckhardt, L. A. and Herzenberg, L. A. (1980). “Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression.” Immunogenetics 11(3): 275-91.Abstract
Howard, F. D., Ledbetter, J. A., Mehdi, S. Q. and Herzenberg, L. A. (1980). “A rapid method for the detection of antibodies to cell surface antigens: a solid phase radioimmunoassay using cell membranes.” Journal of Immunological Methods 38(1-2): 75-84.Abstract
Ledbetter, J. A., Rouse, R. V., Micklem, H. S. and Herzenberg, L. A. (1980). “T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views.” Journal of Experimental Medicine 152(2): 280-95.Abstract
Habu, S., Kasai, M., Nagai, Y., Tamaoki, N., T, T. A., Herzenberg, L. A. and Okumura, K. (1980). “The glycolipid asialo GM1 as a new differentiation antigen of fetal thymocytes.” Journal of Immunology 125(5): 2284-8.Abstract
Parsons, M. and Herzenberg, L. A. (1981). “A monoclonal mouse antiallotype antibody reacts with certain human and other vertebrate immunoglobulins: genetic and phylogenetic findings.” Immunogenetics 12(3-4): 207-19.Abstract
Herzenberg, L. A., J.A. Ledbetter (1981). Homologous T cell differentiation antigens of mouse and man. Frontiers in Immunology. W. E. Hildemann. New York, Elsevier/North-Holland: 179-18.
Howard, F. D., Ledbetter, J. A., Wong, J., Bieber, C. P., Stinson, E. B. and Herzenberg, L. A. (1981). “A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation.” Journal of Immunology 126(6): 2117-22.Abstract
Iverson, G. M., Bianchi, D. W., Cann, H. M. and Herzenberg, L. A. (1981). “Detection and isolation of fetal cells from maternal blood using the flourescence-activated cell sorter (FACS).” Prenatal Diagnosis 1(1): 61-73.Abstract
Haaijman, J. J., Micklem, H. S., Ledbetter, J. A., Dangl, J. L. and Herzenberg, L. A. (1981). “T cell ontogeny. Organ location of maturing populations as defined by surface antigen markers is similar in neonates and adults.” Journal of Experimental Medicine 153(3): 605-14.Abstract
Lipinski, M. and Herzenberg, L. A. (1981). “Les hybridomes et leurs applications.” La Recherche 125: 952-961.
Herzenberg, L. A., T. Tokuhisa, F.T. Gadus, S.J. Black, L.A. Herzenberg (1981). Regulation of memory B cell development and expression. Frontiers in Immunology. W. H. a. F. Hildemann, J.A. New York, Elsevier/North Holland, Inc.: 75-90.
Lanier, L. L., Warner, N. L., Ledbetter, J. A. and Herzenberg, L. A. (1981). “Expression of Lyt-1 antigen on certain murine B cell lymphomas.” Journal of Experimental Medicine 153(4): 998-1003.Abstract
Herzenberg, L. A., T. Takeshi, D.R. Parks, L.A. Herzenberg (1981). Hapten-specific regulation of heterogeneous antibody responses: Intersection of Theory and Practices. The Immune System. Basel, Switzerland, S. AG.
Ledbetter, J. A., Seaman, W. E., Tsu, T. T. and Herzenberg, L. A. (1981). “Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity.” Journal of Experimental Medicine 153(6): 1503-16.Abstract
Herzenberg, L. A., T. Tokuhisa, L.A. Herzenberg (1981). Carrier specific induction of hapten-specific suppression. T Lymphocytes Today. J. Inglis. New York, Elsevier Science: 77-83.
Dowsett, A. P. and Herzenberg, L. A. (1981). “Regulation of the production of murine IgG2a by an H-2-linked gene and other unlinked genes.” Immunogenetics 13(3): 237-45.Abstract
Herzenberg, L. A., T. Tokuhisa, L.A. Herzenberg (1981). Hapten-specific regulation of memory B cell expression. B Lymphocytes in the Immune Response: Functional, Developmental and Interactive Properties. M. D. Klinman N, Scher I, Vittetta E. New York, Elsevier/North Holland: 411-413.
Parsons, M., V. Oi, T. and L. A. Herzenberg (1982). Monoclonal Anti-Allotype Antibodies as Probes of Immunoglobulin Structure. Monoclonal Antibodies and T Cell Hybridomas. H. U. Hammerling GJ, Kearney K
Seaman, W. E., Talal, N., Herzenberg, L. A. and Ledbetter, J. A. (1981). “Surface antigens on mouse natural killer cells: use of monoclonal antibodies to inhibit or to enrich cytotoxic activity.” Journal of Immunology 127(3): 982-6.Abstract
Lipinski, M., Parks, D. R., Rouse, R. V. and Herzenberg, L. A. (1981). “Human trophoblast cell-surface antigens defined by monoclonal antibodies.” Proceedings of the National Academy of Sciences of the United States of America 78(8): 5147-50.Abstract
Ledbetter, J., Frankel AE, Herzenberg LA, Herzenberg LA (1981). Human Leu T cell differentation antigens - Quantitative expression in normal lympoid cells and cell lines. Monoclonal Antibodies and T Cell Hybridomas. H. U. Hammerling GJ, Kearney K. New Yoek, Elsevier/North Holland: 16-22.
Parks, D. R. and Herzenberg, L. A. (1982). “Fetal cells from maternal blood: their selection and prospects for use in prenatal diagnosis.” Methods in Cell Biology 26: 277-95.
Honologous Mouse and Human T Cell Antigens1981Ledbetter, J. A. and L. A. Herzenberg (1981). Honologous Mouse a Human T Cell Antigens. Differentiation Antigens of Lymphocytes. N. L. Warner, Academic Press.
Carrier specific suppression operates through the hapten-specific system1981Herzenberg, L. A., T. Tokuhisa and L. A. Herzenberg (1981). Carrier specific suppression operates through the hapten-specific system. Immunoglobulin Idiotypes and theit Expression, INC-UCLA Symposia on Molecular and Cellular Biology. XX.
Herzenberg, L. A., C.M. Huang, V.T. Oi, M. Parsons (1981). The structure and genetics of mouse immunogobulin heavy chain constant regions defined by monoclonal anti-allotype antibodies. Immunoglobulin Idiotypes and Their Expression: ICN-UCLA Symposia on Molecular and Cellular Biology. S. E. Janeway C, Wigzell H., Fox CF,. New York, Academic Press. XX: 199-208.
Lanier, L. L., Warner, N. L., Ledbetter, J. A. and Herzenberg, L. A. (1981). “Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies.” Journal of Immunology 127(4): 1691-7.Abstract
Tokuhisa, T., Gadus, F. T. and Herzenberg, L. A. (1981). “Monoclonal antibody to an IgD allotype induces a new type of allotype suppression in the mouse.” J. Exp. Med. 154(3): 921-934.Abstract
Ledbetter, J., Tsu TT, Herzenberg LA (1981). Differentiation antigens on spontaneous and transplanted AKR leukemias. Hybridomas in the Diagnosis and Treatment of Cancer. O. MItchell MS.
Parsons, M., Cazenave, P. A. and Herzenberg, L. A. (1981). “Igh-4D, a new allotype at the mouse IgG1 heavy chain locus.” Immunogenetics 14(3-4): 341-4.
Reidler, J., Oi, V. T., Carlsen, W., Vuong, T. M., Pecht, I., Herzenberg, L. A. and Stryer, L. (1982). “Rotational dynamics of monoclonal anti-dansyl immunoglobulins.” Journal of Molecular Biology 158(4): 739-46.
Bakhshi, A., Miyasaka, N., Kavathas, P., Daniels, T. E., Strand, C. V., Herzenberg, L. A. and Talal, N. (1983). “Lymphocyte subsets in Sjogren's syndrome: a quantitative analysis using monoclonal antibodies and the fluorescence-activated cell sorter.” Journal of Clinical & Laboratory Immunology 10(2): 63-9.Abstract
Huber, B. T., Tokuhisa, T. and Herzenberg, L. A. (1981). “Primary and secondary in situ antibody response: abnormal affinity maturation pattern in mice carrying the xid gene.” European Journal of Immunology 11(5): 353-7.Abstract
Herzenberg, L. A., T. Tokuhisa, L.A. Herzenberg (1981). Carrier specific suppression operates through the hapten-specific system. Immunoglobulin Idiotypes and theit Expression, INC-UCLA Symposia on Molecular and Cellular Biology. XX.
Huang, C. M., Parsons, M., Wakeland, E. K., Moriwaki, K. and Herzenberg, L. A. (1982). “New immunoglobulin IgG allotypes and haplotypes found in wild mice with monoclonal anti-allotope antibodies.” Journal of Immunology 128(2): 661-7.Abstract
Frankel, A. E., Rouse, R. V. and Herzenberg, L. A. (1982). “Human prostate specific and shared differentiation antigens defined by monoclonal antibodies.” Proceedings of the National Academy of Sciences of the United States of America 79(3): 903-7.Abstract
Herzenberg, L. A. and Tokuhisa, T. (1982). “Epitope-specific regulation. I. Carrier-specific induction of suppression for IgG anti-hapten antibody responses.” Journal of Experimental Medicine 155(6): 1730-40.
Herzenberg, L. A., Tokuhisa, T. and Parks, D. R. (1982). “Epitope-specific regulation. II. A bistable, Igh-restricted regulatory mechanism central to immunologic memory.” Journal of Experimental Medicine 155(6): 1741-53.
Herzenberg, L. A. and Tokuhisa, T. (1982). “Epitope-specific regulation. III. Induction of allotype-restricted suppression for IgG antibody responses to individual epitopes on complex antigens.” European Journal of Immunology 12(10): 814-8.
Nakai, S., Oi, V., T. and Herzenberg, L. A. (1982). “Nucleotide sequence encoding membrane domains are conserved among immunoglobulin gamma subclass genes.” Biomedical Research 3(1): 37-45.
Dangl, J. L., Parks, D. R., Oi, V. T. and Herzenberg, L. A. (1982). “Rapid isolation of cloned isotype switch variants using fluorescence activated cell sorting.” Cytometry 2(6): 395-401.Abstract
Herzenberg, L. A., Tokuhisa, T. and Hayakawa, K. (1982). “Lack of immune response gene control for induction of epitope-specific suppression by TGAL antigen.” Nature 295(5847): 329-31.
LAH #208
Frankel, A. E., Rouse, R. V., Wang, M. C., Chu, T. M. and Herzenberg, L. A. (1982). “Monoclonal antibodies to a human prostate antigen.” Cancer Research 42(9): 3714-8.Abstract
Hardy, R. R., Hayakawa, K., Haaijman, J. and Herzenberg, L. A. (1982). “B-cell subpopulations identified by two-colour fluorescence analysis.” Nature 297(5867): 589-91.
LAH #210
Herzenberg, L. A. (1983). “Allotype suppression and epitope-specific regulation.” Immunol Today 4(4): 113-117.Abstract
Dangl, J. L. and Herzenberg, L. A. (1982). “Selection of hybridomas and hybridoma variants using the fluorescence activated cell sorter.” Journal of Immunological Methods 52(1): 1-14.
Parks, D. R., Hardy, Richard A., Herzenberg L.A. (1983). “Dual Immunofluorescence - New Frontiers in Cell Analysis and Sorting.” Immunol. Today 4(5): 145-150.
Hardy, R. R., Hayakawa, K., Haaijman, J. and Herzenberg, L. A. (1982). “B-cell subpopulations identifiable by two-color fluorescence analysis using a dual-laser FACS.” Annals of the New York Academy of Sciences 399: 112-21
LAH #217
Herzenberg, L. A. (1982). “Epitope-specific regulation of memory B-cell expression.” Annals of the New York Academy of Sciences 399: 368-74.
Herzenberg, L. A., Hayakawa, K., Hardy, R. R., Tokuhisa, T. and Oi, V. T. (1982). “Molecular, cellular and systemic mechanisms for regulating IgCH expression.” Immunological Reviews 67: 5-31.
Matossian-Rogers, A., Rogers, P. and Herzenberg, L. A. (1982). “Analysis of Ly-6.2-bearing murine lymphocyte subpopulations in relation to the T-lymphocyte markers, Thy-1, Lyt-1, and Lyt-2.” Cellular Immunology 69(1): 91-100.
Matossian-Rogers, A., Rogers, P., Ledbetter, J. A. and Herzenberg, L. A. (1982). “Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6.” Immunogenetics 15(6): 591-9.Abstract
Kavathas, P. and Herzenberg, L. A. (1983). “Amplification of a gene coding for human T-cell differentiation antigen.” Nature 306(5941): 385-7.Abstract
LAH #223
Hensleigh, P. A., Herzenberg, L. A., Lipman, S. H., Malvehy, R. M., Medearis, A. L., Moore, M. H., Sutherland, K. K. and Waters, V. B. (1983). “Transient immunologic effects of betamethasone in human pregnancy after suppression of preterm labor.” American Journal of Reproductive Immunology 4(2): 83-7.Abstract
Kavathas, P. and Herzenberg, L. A. (1983). “Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting.” Proceedings of the National Academy of Sciences of the United States of America 80(2): 524-8.Abstract
Herzenberg, L. A., K. Hayakawa, R.R. Hardy (1982). “2 Color Facs subpopulation of B Lymphocytes.” Medical Tribune 2: 29-32.
Parsons, M., Oi, V. T., Huang, C. M. and Herzenberg, L. A. (1983). “Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies.” Immunogenetics 18(4): 323-34.Abstract
Medearis, A. L., Hensleigh, P. A., Parks, D. R. and Herzenberg, L. A. (1984). “Detection of fetal erythrocytes in maternal blood post partum with the fluorescence-activated cell sorter.” American Journal of Obstetrics & Gynecology 148(3): 290-5.Abstract
Hayakawa, K., Hardy, R. R., Parks, D. R. and Herzenberg, L. A. (1983). “The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.” Journal of Experimental Medicine 157(1): 202-18.Abstract
Hensleigh, P. A., Waters, V. B. and Herzenberg, L. A. (1983). “Human T lymphocyte differentiation antigens: effects of blood sample storage on Leu antibody binding.” Cytometry 3(6): 453-5.Abstract
Zelaschi, D., Newby, C., Parsons, M., van West, B., Cavalli-Sforza, L. L. and Herzenberg, L. A. (1983). “Human immunoglobulin allotypes: previously unrecognized determinants and alleles defined with monoclonal antibodies.” Proceedings of the National Academy of Sciences of the United States of America 80(12): 3762-6.Abstract
Oi, V. T., Morrison, S. L., Herzenberg, L. A. and Berg, P. (1983). “Immunoglobulin gene expression in transformed lymphoid cells.” Proceedings of the National Academy of Sciences of the United States of America 80(3): 825-9.Abstract
Herzenberg, L. A., Tokuhisa, T. and Hayakawa, K. (1983). “Epitope-specific regulation.” Annual Review of Immunology 1: 609-32.
LAH #233
Haaijman, J. J., Hardy, R. R., Hayakawa, K. and Herzenberg, L. A. (1982). “Distinct B Cell Subpopulations in Mice as characterized by multiparameter fluorescence activated cell sorter (FACS) analysis.” Journal of Experimental Medicine Submitted.
Hardy, R. R., Hayakawa, K., Parks, D. R. and Herzenberg, L. A. (1983). “Demonstration of B-cell maturation in X-linked immunodeficient mice by simultaneous three-colour immunofluorescence.” Nature 306(5940): 270-2.Abstract
Schroder, J., Nikinmaa, B., Kavathas, P. and Herzenberg, L. A. (1983). “Fluorescence-activated cell sorting of mouse-human hybrid cells aids in locating the gene for the Leu 7 (HNK-1) antigen to human chromosome 11.” Proceedings of the National Academy of Sciences of the United States of America 80(11): 3421-4.Abstract
Huang, C. M., Parsons, M., Oi, V. T., Huang, H. J. and Herzenberg, L. A. (1983). “Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies.” Immunogenetics 18(4): 311-21.Abstract
Lanier, L. L., Engleman, E. G., Gatenby, P., Babcock, G. F., Warner, N. L. and Herzenberg, L. A. (1983). “Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry.” Immunological Reviews 74: 143-60.
Oi, V. T., Herzenberg, L. A. and Birshtein, B. K. (1983). “Localization of murine Igh-1a allotypic determinants by using a panel of mouse myeloma variant immunoglobulins.” Journal of Immunology 130(4): 1967-9.Abstract
Parham, P., Kipps, T. J., Ward, F. E. and Herzenberg, L. A. (1983). “Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to cytotoxic IgG2 antibodies.” Human Immunology 8(2): 141-51.Abstract
Oi, V. T., Vuong, T. M., Hardy, R., Reidler, J., Dangle, J., Herzenberg, L. A. and Stryer, L. (1984). “Correlation between segmental flexibility and effector function of antibodies.” Nature 307(5947): 136-40.Abstract
Hayakawa, K., Hardy, R. R., Herzenberg, L. A., Steinberg, A. D. and Herzenberg, L. A. (1984). ly-1 B: Afunctionally distinct B-cell subpopulation. Progress of Immunology V. Tokyo, Academic Press. V: 661-668.
Alberti, S., Stovel, R. and Herzenberg, L. A. (1984). “Preservation of cells sorted individually onto microscope slides with a fluorescence-activated cell sorter.” Cytometry 5(6): 644-7.Abstract
Waldor, M. K., Hardy, R. R., Hayakawa, K., Steinman, L. and Herzenberg, L. A. (1984). “Disappearance and reappearance of B cells after in vivo treatment with monoclonal anti-I-A antibodies.” Proceedings of the National Academy of Sciences of the United States of America 81(9): 2855-8.Abstract
Herzenberg, L. A. (1983). Epitope-Specific Regulation: A Brief Overview. Progress in Immunology V. Japan, Academic Press Japan, Inc. V: 581-589.
Parks, D. R., Hardy, R. R. and Herzenberg, L. A. (1984). “Three-color immunofluorescence analysis of mouse B-lymphocyte subpopulations.” Cytometry 5(2): 159-68.Abstract
LAH #246-01
Parks, D. R. and Herzenberg, L. A. (1984). “Fluorescence-activated cell sorting: theory, experimental optimization, and applications in lymphoid cell biology.” Methods in Enzymology 108: 197-241.
Herzenberg, L. A., P. Kavathis (1984). Transformation and amplification of genes for human differential antigens in mouse fibroblasts,. Immunogenetics: Its Application to Clinical Medicine. Tokyo, Academic Press Japan, Inc.: 283-284.
Hsu, C., Kavathas, P. and Herzenberg, L. A. (1984). “Cell-surface antigens expressed on L-cells transfected with whole DNA from non-expressing and expressing cells.” Nature 312(5989): 68-9.Abstract
Kawata, M., Sizer, K., Sekiya, S., Parnes, J. R. and Herzenberg, L. A. (1984). “Limitation of differential expression of HLA-A,B,C antigens on choriocarcinoma cell lines by messenger RNA for HLA heavy chain but not by beta 2-microglobulin.” Cancer Research 44(9): 4011-6.Abstract
Kawata, M., Parnes, J. R. and Herzenberg, L. A. (1984). “Transcriptional control of HLA-A,B,C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter.” Journal of Experimental Medicine 160(3): 633-51.Abstract
Hardy, R. R., Hayakawa, K., Parks, D. R. and Herzenberg, L. A. (1984). “Murine B cell differentiation lineages.” Journal of Experimental Medicine 159(4): 1169-88.Abstract
Hayakawa, K., Hardy, R. R., Honda, M., Herzenberg, L. A. and Steinberg, A. D. (1984). “Ly-1 B cells: functionally distinct lymphocytes that secrete IgM autoantibodies.” Proceedings of the National Academy of Sciences of the United States of America 81(8): 2494-8.Abstract
Oi, V. T., Hsu, C., Hardy, R. R. and Herzenberg, L. A. (1984). Hybridoma Antibody-Producing Switch Variants: A Variant Lacking the CH1 Domain. Cell Fusion: Gene Transfer and Transformation. R. F. B. E. G. Bassett. New Yok, Raven Press: 281-287.
Kipps, T. J. and Herzenberg, L. A. (1986). Hybridoma immunoglobulin isotype switch variant selection using the fluorescence activated cell sorter. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 4; Applications of immunological methods in biomedical sciences: 109.1-109.8.Abstract
More recently, the authors and others have used the FACS to isolate isotype switch variant hybridomas producing monoclonal antibodies with selected specifities and desired functional activities. Experiments have shown that spontaneous immunoglobulin gene switching occurs in hybridomas with frequencies similar to those noted with cultured myeloma cells. Furthermore, the binding specificity of the switched monoclonal antibody is identical to that of the original parent immunoglobulin molecule. Thus, by defining the methodology required for switch variant selection, a hybridoma clone producing a monoclonal antibody with a desired specificity may be modified to secrete antigen-specific immunoglobulin with an isotype of desired biological activity.
The ability to select switch variant hybridomas producing monoclonal antibody with a particular immunoglobulin isotype may greatly enhance the application of monoclonal antibody technology. Even among the subclasses of mouse IgG -- namely IgG1, IgG2a, and IgG3 -- major differences exist. Comparison of the segmental flexibility using nanosecond fluorescence spectroscopy has demonstrated IgG2b to be most flexible, IgG2a slightly less flexible and IgG1 relatively rigid. Furthermore, each immunoglobulin subclass possess discrete biological activity. IgG2a and IgG2b fix guinea-pig and rabbit complement through the conventional pathway, whereas IgG1 and IgG3 do not. IgG1 molecules, but not IgG2a, IgG2b, or IgG3, are capable of mediating passive cutaneous anaphylactic (PCA) reactions in the mouse. IgG2a and IgG2b subclasses, but not IgG1, mediate the same reaction in the guinea-pig. More recently, the authors have detected differences between these three classes of murine immunoglobulin in their ability to participate in human antibody-dependent cellular cytotoxicity (ADCC). IgG2a functions better than IgG2b in human ADCC, whereas IgG1 is inactive. Furthermore, mouse immunoglobulin isotypes of anti-tumour antibodies apparently differ in their ability to influence tumour growth in experimental animal model systems. Thus the isotype of a given monoclonal antibody may play a critical role in its biological effector function. The ability to select switch variant cells from a hybridoma originally seleted for the binding specificity of its secreted monoclonal antibody may allow for the produciton of specific monoclonals with isotypes of desired biological activity.
Since 1978, the authors have simplified and improved the process of switch variant selection using theFACS. The first switch variants were isolated only after repeated rounds of FACS variant selection, involving months of tissue culture. Currently, it is possible to detect and, in most cases, directly clone switch variant cells with only one round of FACS selection. Here the authors describe these advances which greatly facilitate the process of switch variant selection.
LAH #256
Hardy, R. R., Hayakawa, K., Herzenberg, L. A., Morse, H. C. d. and Davidson, W. F. (1984). “Ly-1 as a differentiation antigen on normal and neoplastic B cells.” Current Topics in Microbiology & Immunology 113: 231-6.
Kavathas, P., Sukhatme, V. P., Herzenberg, L. A. and Parnes, J. R. (1984). “Isolation of the gene encoding the human T-lymphocyte differentiation antigen Leu-2 (T8) by gene transfer and cDNA subtraction.” Proceedings of the National Academy of Sciences of the United States of America 81(24): 7688-92.Abstract
Kipps, T. J., and Leonard A. Herzenberg (1986). Schemata for the production of monoclonal antibody producing hybridomas. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 4; Applications of immunological methods in biomedical sciences: 108.1-108.9.
Kavathas, P. a. L. A. H. (1986). Transfection for lymphocyte cell surface antigens. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 3; Genetics and Molecular Immunology: 91.1 - 91.10.
LAH #259-01
Kavathas, P. (1986). Amplification and molecular cloning of transfected genes. Hybridomas in Biotechnology and Medicine. T. A. Springer, Plenum Press. N/A.
Zelaschi, D., M.J. Johnson, C.J. Newby and Leonore A. Herzenberg (1986). New methods for human typing. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 3; Genetics and Molecular Immunology: 95.1 - 95.10.
Hardy, R. R. (1986). Purification and Characterization of Monoclonal Antibodies. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 1; Immunochemistry: 13.1
Hardy, R. R. (1986). Complement fixation by defined antibody-antigen complexes. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 1; Immunochemistry: 40.1
Hardy, R. R. (1986). Purification and coupling of fluorescent proteins for use in flow cytometry. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 1; Immunochemistry: 31.1 - 31.12.
Herzenberg, L. A. (1986). A brief overview: Epitope-specific regulation. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell, L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 2; Cellular Immunology: 74.1 -.
LAH #263
Parsons, M., Leonore A. Herzenbeg, Alan M. Stall and Leonard A. Herzenberg (1986). Mouse immunoglobulin allotypes: description and special methodology. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 3; Genetics and Molecular Immunology: 97.1 - 97.17.
LAH #264
Newby, C. J. and Herzenberg, L. A. H., K. (1986). Solid-phase radio-immunoassaya. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell, and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publication, Ltd. 1; Immunochemistry: 34.1 -.
LAH #265
Parks, D., Lanier, L. and Herzenberg, L. A. (1986). Flow cytometry and fluorescence activated cell sorting. The Handbook of Experimental Immunology. L. A. Herzenberg, D. M. Weir, C. C. Blackwell and L. A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 1 Immunoochemisry: 291-29.21.
Moore, W. and Kautz, R. (1986). Data analysis for flow cytometry. The Handbook of Experimental Immunology. L. A. H. D.M. Weir, C.C. Blackwell, and L.A. Herzenberg. Edinburgh, Blackwell Scientific Publications, Ltd. 1; Immunochemistry: 30.1 -.
Kipps, T. J. and Herzenberg, L. A. (1985). Fluorescence activated cell sorter and monoclonal antibodies: complementary tools in immunodiagnosis and immuntherapy. Rapid methods and automation in microbiology and immunology. Habermehl. Heidelberg, Springer Verlag. One: 115 - 122.
Herzenberg, L. A., Hsu, C., Alberti, S. and Kavathas, P. (1984). “Transfection and cloning of genes for membrane antigens using the FACS.” Medical Oncology & Tumor Pharmacotherapy 1(4): 219-24.Abstract
Morrison, S. L., Johnson, M. J., Herzenberg, L. A. and Oi, V. T. (1984). “Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.” Proceedings of the National Academy of Sciences of the United States of America 81(21): 6851-5.Abstract
Tagawa, M., Tokuhisa, T., Ono, K., Taniguchi, M. and Herzenberg, L. A. (1984). “Epitope-specific regulation. IV. In vitro studies with suppressor T cells induced by carrier/hapten-carrier immunization.” Cellular Immunology 86(2): 327-36.Abstract
Waldor, M. K., Sriram, S., Hardy, R., Herzenberg, L. A., Lanier, L., Lim, M. and Steinman, L. (1985). “Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker.” Science 227(4685): 415-7.Abstract
LAH #272
Kipps, T. J., Parham, P., Punt, J. and Herzenberg, L. A. (1985). "Importance of Immunolglobulin Isotype in Human Antibody-Dependent, Cell-Mediated Cytotoxicity Directed by Murine Monoclonal Antibodies." Journal of Experimental Medicine 161: 1-17
LAH #272-03
Kipps, T. J. (1985). Switching the isotype of monoclonal antibodies. Hybridoma Technology in theBiosciences and Medicine. T. Springer. New York, Plenum Press. One: 89-101.
Parham, P., Antonelli, P., Herzenberg, L. A., Kipps, T. J., Fuller, A. and Ward, F. E. (1986). “Further studies on the epitopes of HLA-B7 defined by murine monoclonal antibodies.” Human Immunology 15(1): 44-67.Abstract
Suomalainen, H. A., Herzenberg, L. A., Gahmberg, C. G., Sussman, H. H. and Schroder, J. (1985). “Assignment of gene for human cell-surface membrane antigen Trop-4 to chromosome 11.” Somatic Cell & Molecular Genetics 11(3): 257-65.Abstract
Kipps, T. J. and Herzenberg, L. A. (1986). “Homologous chromosome recombination generating immunoglobulin allotype and isotype switch variants.” EMBO Journal 5(2): 263-8.Abstract
Hayakawa, K., Hardy, R. R. and Herzenberg, L. A. (1985). “Progenitors for Ly-1 B cells are distinct from progenitors for other B cells.” Journal of Experimental Medicine 161(6): 1554-68.Abstract
Hardy, R. R., Dangl, J. L., Hayakawa, K., Jager, G. and Herzenberg, L. A. (1986). “Frequent lambda light chain gene rearrangement and expression in a Ly-1 B lymphoma with a productive kappa chain allele.” Proceedings of the National Academy of Sciences of the United States of America 83(5): 1438-42.Abstract
Sidman, C. L., Shultz, L. D., Hardy, R. R., Hayakawa, K. and Herzenberg, L. A. (1986). “Production of immunoglobulin isotypes by Ly-1+ B cells in viable motheaten and normal mice.” Science 232(4756): 1423-5.Abstract
LAH #279-01
Bowcock, A. M., Kavathas, P., Margolskee, R. F., Herzenberg, L. and Cavalli-Sforza, L. L. (1986). “An RFLP associated with pcDLeu2-14, a human T-cell differentiation antigen CD8 (Leu2) cDNA mapped to 2p12.” Nucleic Acids Research 14(19): 7817.
Herzenberg, L. A., Kipps, T. J., Peterson, L. and Parks, D. R. (1985). Hybridoma variants affecting isotype, antigen binding and idiotype. Biotechnology in Diagnostics (Proceedings of the International Symposium on the Impact of Biotechnology on Diagnostics). H. Koprowski, S. Ferrone and A. Albertini. Amsterdam, Elsevier Science Publications. One: 3-16.
Nakauchi, H., Nolan, G. P., Hsu, C., Huang, H. S., Kavathas, P. and Herzenberg, L. A. (1985). “Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions.” Proceedings of the National Academy of Sciences of the United States of America 82(15): 5126-30.Abstract
Bruns, G., Kavathas, P., Shiloh, Y., Sakai, K., Schwaber, J., Latt, S. A. and Herzenberg, L. A. (1985). “The human T cell antigen Leu-2 (T8) is encoded on chromosome 2.” Human Genetics 70(4): 311-4.Abstract
Herzenberg, L. A., Stall, A. and Lalor, P. (1987). Ly-1 B cells and autoimmunity. New Horizons in Animal Models for Autoimmune Disease, Academic Press: 149-157.
Hayakawa, K., Hardy, R. R. and Herzenberg, L. A. (1986). “Peritoneal Ly-1 B cells: genetic control, autoantibody production, increased lambda light chain expression.” European Journal of Immunology 16(4): 450-6.Abstract
Tagawa, M., Nakauchi, H., Herzenberg, L. A. and Nolan, G. P. (1986). “Formal proof that different-size Lyt-2 polypeptides arise from differential splicing and post-transcriptional regulation.” Proceedings of the National Academy of Sciences of the United States of America 83(10): 3422-6.Abstract
Stall, A. M., Lalor, P. A., Herzenberg, L. A. and Herzenberg, L. A. (1986). “Ly-1 B cells and autoantibodies.” Annales d'Immunologie de l'Institut Pasteur 137D: 173-175.
Herzenberg, L. A., Stall, A. M., Lalor, P. A., Sidman, C., Moore, W. A. and Parks, D. R. (1986). “The Ly-1 B cell lineage.” Immunological Reviews 93: 81-102.
Alberti, S. and Herzenberg, L. A. (1986). Transfection of DNA from choriocarcinoma cell lines and sperm cells: DNA methylation prevents the expression of genes for the major histocompatibility complex (HLA) class I andthe T cell differentiation antigen, Leu-2. Reproductive Immunology 1986 (Proceedings of the Third International Congress of Reproductive Immunology, Toronto, July 1986). Amsterdam, Elsevier Science Publishers: 60-66.
Alberti, S. and Herzenberg, L. A. (1986). “DNA transfection: Gene regulation, gene amplificatoin and gene cloning.” Juntendo Med Jnl 32: 423-425.
Huang, H. J., Jones, N. H., Strominger, J. L. and Herzenberg, L. A. (1987). “Molecular cloning of Ly-1, a membrane glycoprotein of mouse T lymphocytes and a subset of B cells: molecular homology to its human counterpart Leu-1/T1 (CD5).” Proceedings of the National Academy of Sciences of the United States of America 84(1): 204-8.Abstract
Herzenberg, L. A., Lalor, P., Stall, A. M., Tarlinton, D. and Herzenberg, L. A. (1986). Characteristics of Ly-1 B cells. Progress in Immunology VI (6th Intl. Congress of Immunology, July 1986, Toronto). B. Cinader and R. G. Miller. Orlando, Academic Press: 376-384.
Kleinfield, R., Hardy, R. R., Tarlinton, D., Dangl, J., Herzenberg, L. A. and Weigert, M. (1986). “Recombination between an expressed immunoglobulin heavy-chain gene and a germline variable gene segment in a Ly 1+ B-cell lymphoma.” Nature 322(6082): 843-6.Abstract
Lalor, P. A. and Herzenberg, L. A. (1987). “Transfection of genes encoding lymphocyte differentiation antigens: applications in veterinary immunology.” Veterinary Immunology & Immunopathology 17(1-4): 291-302.Abstract
Hayakawa, K., Hardy, R. R., Stall, A. M. and Herzenberg, L. A. (1986). “Immunoglobulin-bearing B cells reconstitute and maintain the murine Ly-1 B cell lineage.” European Journal of Immunology 16(10): 1313-6.Abstract
Steinman, L., Waldor, M. K., Zamvil, S. S., Lim, M., Herzenberg, L., McDevitt, H. O., Mitchell, D. and Sriram, S. (1986). “Therapy of autoimmune diseases with antibody to immune response gene products or to T-cell surface markers.” Annals of the New York Academy of Sciences 475: 274-84.
LAH #294
Alberti, S., Parks, D. R. and Herzenberg, L. A. (1987). “A single laser method for subtraction of cell autofluorescence in flow cytometry.” Cytometry 8(2): 114-9.Abstract
Radeke, M. J., Misko, T. P., Hsu, C., Herzenberg, L. A. and Shooter, E. M. (1987). “Gene transfer and molecular cloning of the rat nerve growth factor receptor.” Nature 325(6105): 593-7.
Hagiwara, H., Huang, H. J., Arai, N., Herzenberg, L. A., Arai, K. and Zlotnik, A. (1987). “Interleukin 1 modulates messenger RNA levels of lymphokines and of other molecules associated with T cell activation in the T cell lymphoma LBRM33-1A5.” Journal of Immunology 138(8): 2514-9.Abstract
Nakauchi, H., Tagawa, M., Nolan, G. P. and Herzenberg, L. A. (1987). “Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2.” Nucleic Acids Research 15(10): 4337-47.Abstract
Jones, N. H., Clabby, M. L., Dialynas, D. P., Huang, H. J., Herzenberg, L. A. and Strominger, J. L. (1986). “Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1.” Nature 323(6086): 346-9.Abstract
Herzenberg, L. A. (1987). Digital processing and immuno-regulatory circuits. The Semiotics of Cellular Communication in the Immune System, Springer Verlag.
Herzenberg, L. A., Stall, A. M., Braun, J., Weaver, D., Baltimore, D., Herzenberg, L. A. and Grosschedl, R. (1987). “Development of the predominant B cell lineage is imparied in immunoglobulin heavy chain transgenic mice.” Nature 329: 71-73.
Waldor, M. K., Mitchell, D., Kipps, T. J., Herzenberg, L. A. and Steinman, L. (1987). “Importance of immunoglobulin isotype in therapy of experimental autoimmune encephalomyelitis with monoclonal anti-CD4 antibody.” Journal of Immunology 139(11): 3660-4.Abstract
Panka, D. J., Mudgett-Hunter, M., Parks, D. R., Peterson, L. L., Herzenberg, L. A., Haber, E. and Margolies, M. N. (1988). “Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies.” Proceedings of the National Academy of Sciences of the United States of America 85(9): 3080-4.Abstract
Nolan, G. P., Fiering, S., Nicolas, J. F. and Herzenberg, L. A. (1988). “Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ.” Proceedings of the National Academy of Sciences of the United States of America 85(8): 2603-7.Abstract
Stall, A. M., Kroese, F. G., Gadus, F. T., Sieckmann, D. G. and Herzenberg, L. A. (1988). “Rearrangement and expression of endogenous immunoglobulin genes occur in many murine B cells expressing transgenic membrane IgM.” Proceedings of the National Academy of Sciences of the United States of America 85(10): 3546-50.Abstract
Nakauchi, H., Nolan, G. P., Tagawa, M. and Herzenberg, L. A. (1987). Cloning, sequencing and differential splicing of the Lyt-2 gene. New Horizons in Animal Models for Autoimmune Disease, Academic Press: 133-140.
Dangl, J. L., Wensel, T. G., Morrison, S. L., Stryer, L., Herzenberg, L. A. and Oi, V. T. (1988). “Segmental flexibility and complement fixation of genetically engineered chimeric human, rabbit and mouse antibodies.” EMBO Journal 7(7): 1989-94.Abstract
Stall, A. M., Farinas, M. C., Tarlinton, D. M., Lalor, P. A., Herzenberg, L. A. and Strober, S. (1988). “Ly-1 B-cell clones similar to human chronic lymphocytic leukemias routinely develop in older normal mice and young autoimmune (New Zealand Black-related) animals.” Proceedings of the National Academy of Sciences of the United States of America 85(19): 7312-6.Abstract
LAH #309
Kroese, F. G., Butcher, E. C., Stall, A. M. and Herzenberg, L. A. (1989). “A major peritoneal reservoir of precursors for intestinal IgA plasma cells.” Immunological Investigations 18(1-4): 47-58.Abstract
Alberti, S. and Herzenberg, L. A. (1988). “DNA methylation prevents transfection of genes for specific surface antigens.” Proceedings of the National Academy of Sciences of the United States of America 85(22): 8391-4.Abstract
Tarlinton, D., Stall, A. M. and Herzenberg, L. A. (1988). “Repetitive usage of immunoglobulin VH and D gene segments in CD5+ Ly-1 B clones of (NZB x NZW)F1 mice.” EMBO Journal 7(12): 3705-10.Abstract
Kerr, W. G., Nolan, G. P. and Herzenberg, L. A. (1991). In situ detection of transcriptionally-active chromatin and genetic regulatory elements in individual viable mammalian cells. Mechanisms of Lymphocyte Activation and Immune Regulation III. S. e. a. Gupta. New York, Plenum Press: 187-200.
LAH #312-01
Kerr, W. G., Nolan, G. P. and Herzenberg, L. A. (1989). “In situ detection of transcriptionally active chromatin and genetic regulatory elements in individual viable mammalian cells.” Immunology. Supplement 2: 74-8; discussion 79.Abstract
Lalor, P. A., Stall, A. M., Adams, S. and Herzenberg, L. A. (1989). “Permanent alteration of the murine Ly-1 B repertoire due to selective depletion of Ly-1 B cells in neonatal animals.” European Journal of Immunology 19(3): 501-6.Abstract
Lalor, P. A., Herzenberg, L. A., Adams, S. and Stall, A. M. (1989). “Feedback regulation of murine Ly-1 B cell development.” European Journal of Immunology 19(3): 507-13.Abstract
Kroese, F. G., Butcher, E. C., Stall, A. M., Lalor, P. A., Adams, S. and Herzenberg, L. A. (1989). “Many of the IgA producing plasma cells in murine gut are derived from self-replenishing precursors in the peritoneal cavity.” International Immunology 1(1): 75-84.Abstract
Parks, D. R., Herzenberg, L. A. and Herzenberg, L. A. (1989). Flow cytometry and fluorescence-activated cell sorting. Fundamental Immunology. W. E. Paul. New York, Raven Press: 781-802.
Herzenberg, L. A., Lalor, P. A. and Stall, A. M. (1989). “Are Ly-1 B cells important in autoimmune disease?” Journal of Autoimmunity 2 Suppl: 225-31.Abstract
Yancopoulos, G. D., Nolan, G. P., Pollock, R., Prockop, S., Li, S. C., Herzenberg, L. A. and Alt, F. W. (1989). Lymphoid VDJ recombinase activity: Development of a novel fluorescence-based assay system. Vectors as Tools for the Study ofNormal and Abnormal Growth and Differentiation. H. e. a. Lother. Berlin and Heidelberg, Springer Verlag. 34.
Jutila, M. A., Kroese, F. G., Jutila, K. L., Stall, A. M., Fiering, S., Herzenberg, L. A., Berg, E. L. and Butcher, E. C. (1988). “Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma.” European Journal of Immunology 18(11): 1819-26.Abstract
Herzenberg, L. A. (1989). “Toward a layered immune system.” Cell 59(6): 953-4.
Leclerc, C. and Herzenberg, L. A. (1989). “Regulation of antibody production by suppressor T cells.” Res. Immunol 140: 285-345.
LAH #321-01
Herzenberg, L. A. (1989). “Murine suppressor T cells: mirage or cloudy reality?” Research in Immunology 140(3): 337-8; discussion 339-45.
Kerr, W. G., Nolan, G. P., Serafini, A. T. and Herzenberg, L. A. (1989). “Transcriptionally defective retroviruses containing lacZ for the in situ detection of endogenous genes and developmentally regulated chromatin.” Cold Spring Harbor Symposia on Quantitative Biology 54 Pt 2: 767-76.
Herzenberg, L. A. and Stall, A. M. (1989). “Conventional and Ly-1 B-cell lineages in normal and mu transgenic mice.” Cold Spring Harbor Symposia on Quantitative Biology 54 Pt 1: 219-25.
LAH #324
Herzenberg, L. A. and Stall, A. (1989). B cell lineages and immunologic memory. Progress in Immunology VIII. F. Melchers.
Yancopoulos, G. D., Nolan, G. P., Pollock, R., Prockop, S., Li, S. C., Herzenberg, L. A. and Alt, F. W. (1990). “A novel fluorescence-based system for assaying and separating live cells according to VDJ recombinase activity.” Molecular & Cellular Biology 10(4): 1697-704.Abstract
Farinas, M. C., Stall, A. M., Solovera, J. J., Tarlinton, D. M., Herzenberg, L. A. and Strober, S. (1990). “Ly-1 B cells and disease activity in (New Zealand black x New Zealand white)F1 mice. Effect of total lymphoid irradiation.” Arthritis & Rheumatism 33(4): 553-62.Abstract
Portnoi, D., Stall, A. M., Schwartz, D., Merigan, T. C., Herzenberg, L. A. and Basham, T. (1990). “Zidovudine (azido dideoxythymidine) inhibits characteristic early alterations of lymphoid cell populations in retrovirus-induced murine AIDS.” Journal of Immunology 144(5): 1705-10.Abstract
Fiering, S., Northrop, J. P., Nolan, G. P., Mattila, P. S., Crabtree, G. R. and Herzenberg, L. A. (1990). “Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.” Genes & Development 4(10): 1823-34.Abstract
Kerr, W. G., Nolan, G. P., Johnsen, J. B. and Herzenberg, L. A. (1991). “In situ detection of stage-specific genes and enhancers in B cell differentiation via gene-search retroviruses.” Advances in Experimental Medicine & Biology 292: 187-200.Abstract
Chen, J. Z., Stall, A. M. and Herzenberg, L. A. (1990). “Differences in glycoprotein complexes associated with IgM and IgD on normal murine B cells potentially enable transduction of different signals.” EMBO Journal 9(7): 2117-24.Abstract
Kipps, T. J., Parham, P., Punt, J. and Herzenberg, L. A. (1985). “Importance of immunoglobulin isotype in human antibody-dependent, cell-mediated cytotoxicity directed by murine monoclonal antibodies.” Journal of Experimental Medicine 161(1): 1-17.Abstract
Chen, J. and Herzenberg, L. A. (1991). “Heparin alters the expression of different forms of immunoglobulin mu heavy chains and their associated proteins by pre-B cell lines and normal Ly-1 (CD5+) B cells.” International Immunology 3(11): 1117-27.Abstract
LAH #332
Kroese, F. G., Butcher, E. C., Lalor, P. A., Stall, A. M. and Herzenberg, L. A. (1990). “The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations.” European Journal of Immunology 20(7): 1527-34.Abstract
Roederer, M., Staal, F. J., Raju, P. A., Ela, S. W. and Herzenberg, L. A. (1990). “Cytokine-stimulated human immunodeficiency virus replication is inhibited by N-acetyl-L-cysteine.” Proceedings of the National Academy of Sciences of the United States of America 87(12): 4884-8.Abstract
Nawata, Y., Stall, A. M., Herzenberg, L. A., Eugui, E. M. and Allison, A. C. (1990). “Surface immunoglobulin ligands and cytokines differentially affect proliferation and antibody production by human CD5+ and CD5- B lymphocytes.” International Immunology 2(7): 603-14.Abstract
LAH #336
Weir, D. M., Herzenberg, L. A., Blackwell, C. C. and Herzenberg, L. A., Eds. (1986). The Handbook of Experimental Immunology. The Handbook of Experimental Immunology. Edinburgh, Blackwell Scientific Publications, Ltd.
MacGregor, G. R., Nolan, G. P., Fiering, S., Roederer, M. and Herzenberg, L. A. (1991). Use of E. coli lacZ (b-Galactosidase) as a reporter gene. Methods in Molecular Biology. E. J. Murray. Clifton, New Jersey, The Humana Press Inc. 7; Gene Transfer and Expression Protocols: 217-230.
Barsalou, T., Sujansky, W., Herzenberg, L. A. and Wiederhold, G. (1991). “Management of complex immunogenetics information using an enhanced relational model.” Computers & Biomedical Research 24(5): 476-98.Abstract
Schildbach, J. F., Panka, D. J., Parks, D. R., Jager, G. C., Novotny, J., Herzenberg, L. A., Mudgett-Hunter, M., Bruccoleri, R. E., Haber, E. and Margolies, M. N. (1991). “Altered hapten recognition by two anti-digoxin hybridoma variants due to variable region point mutations.” Journal of Biological Chemistry 266(7): 4640-7.Abstract
Krasnow, M. A., Cumberledge, S., Manning, G., Herzenberg, L. A. and Nolan, G. P. (1991). “Whole animal cell sorting of Drosophila embryos.” Science 251(4989): 81-5.Abstract
Bierer, B. E., Mattila, P. S., Standaert, R. F., Herzenberg, L. A., Burakoff, S. J., Crabtree, G. and Schreiber, S. L. (1990). “Two distinct signal transmission pathways in T lymphocytes are inhibited by complexes formed between an immunophilin and either FK506 or rapamycin.” Proceedings of the National Academy of Sciences of the United States of America 87(23): 9231-5.Abstract
Mattila, P. S., Ullman, K. S., Fiering, S., Emmel, E. A., McCutcheon, M., Crabtree, G. R. and Herzenberg, L. A. (1990). “The actions of cyclosporin A and FK506 suggest a novel step in the activation of T lymphocytes.” EMBO Journal 9(13): 4425-33.Abstract
LAH #343
Kroese, F. G. M., Hermans, M. H., de Boer, N. K., Butcher, E. C. and Herzenberg, L. A. (1991). Rat B cell subsets identified by flow-cytometry and their anatomical ounterparts. Lymphatic Tissues and In Vivo Immune Responses. S. Ezine, S. Berrih-Aknin and B. Imhof. New York, Marcel Dekker.
LAH #344
Russo-Marie, F., Roederer, M., Sager, B., Herzenberg, L. A. and Kaiser, D. (1993). "Beta-galactosidase activity in single differentiating bacterial cells." Proceedings of the National Academy of Sciences of the United States of America 90(17): 8194-8..Abstract
Staal, F. J., Roederer, M. and Herzenberg, L. A. (1990). “Intracellular thiols regulate activation of nuclear factor kappa B and transcription of human immunodeficiency virus.” Proceedings of the National Academy of Sciences of the United States of America 87(24): 9943-7.Abstract
Roederer, M., Raju, P. A., Staal, F. J. and Herzenberg, L. A. (1991). “N-acetylcysteine inhibits latent HIV expression in chronically infected cells.” AIDS Research & Human Retroviruses 7(6): 563-7.Abstract
Fiering, S. N., Roederer, M., Nolan, G. P., Micklem, D. R., Parks, D. R. and Herzenberg, L. A. (1991). “Improved FACS-Gal: flow cytometric analysis and sorting of viable eukaryotic cells expressing reporter gene constructs.” Cytometry 12(4): 291-301.Abstract
Roederer, M., Fiering, S. and Herzenberg, L. A. (1991). “FACS-Gal: Flow cytometric analysis and sorting of cells expressing reporter gene constructs.” Methods: A Companion to Meth. Enzymol. 2(3 (June)): 248-260.
Herzenberg, L. A., Kantor, Aaron B., Herzenberg, Leonard A., Parks, David R. (1992). Flow cytometry. Encyclopedia of Immunology. I. M. Roitt and P. J. Delves. London, Academic Press. 2: 576-581.
Roederer, M., Staal, F.J.T., Raju, P.A., Herzenberg, L.A., Herzenberg, L.A. (1992). The Interrelationship of Tumor Necrosis Factor, Glutathione, and AIDS. Tumor Necrosis Factor: Structure-Function Relationship and Clinical Application. (3rd International Conference on Tumor Necrosis Factor and Related Cytokines, November 21-25, 1990, Makuhari, Chiba, Japan). T. Osawa and B. Bonavida. Basel, S. Karger, AG: 215-229.
Roederer, M., Staal, F. J., Osada, H. and Herzenberg, L. A. (1991). “CD4 and CD8 T cells with high intracellular glutathione levels are selectively lost as the HIV infection progresses.” International Immunology 3(9): 933-7.Abstract
Kerr, W. and Herzenberg, L. A. (1991). Gene-search viruses and FACs Fall permit the detection, isolation and characterization of mammalian cells with in situ fusions between cellular genes and E.coli lacZ. Methods: A Companion Volume to Methods in Enzymology, Academic Press. 2(3): 261-271.
Staal, F. J. T., Roederer, M., Israelski, D. M., Bubp, J., Mole, L. A., McShane, D., Deresinski, S. C., Ross, W., Sussman, H., Raju, P. A., Anderson, M. T., Moore, W., Ela, S. W., Herzenberg, L. A. and Herzenberg, L. A. (1992). “Intracellular glutathione levels in T cell subsets decrease in HIV infected individuals.” AIDS Res. Human Retrov. 8(2): 305-314.Abstract
LAH #354
Staal, F. J., Roederer, M., Bubp, J., Mole, L. A., Anderson, M. T., Raju, P. A., Israelski, D. M., Deresinski, S. C., Moore, W. A., Herzenberg, L. A. and et al. (1992). “CD20 expression is increased on B lymphocytes from HIV-infected individuals [published erratum appears in J Acquir Immune Defic Syndr 1992;5(9):956].” J Acquir Immune Defic Syndr 5(6): 627-32.Abstract
Kantor, A. B., Stall, A. M., Adams, S. and Herzenberg, L. A. (1992). “Differential development of progenitor activity for three B-cell lineages.” Proceedings of the National Academy of Sciences of the United States of America 89(8): 3320-4.Abstract
Staal, F. J., Roederer, M. and Herzenberg, L. A. (1992). “Glutathione and immunophenotypes of T and B lymphocytes in HIV-infected individuals.” Annals of the New York Academy of Sciences 651: 453-63.
LAH #356-01
Herzenberg, L. A., Haughton, G. and Rajewsky, K., Eds. (1992). CD5 B Cells in Development and Disease. Annals of the N.Y. Acad. Sci. NY, N.Y. Acad. Sci.
Kantor, A. B. (1992). “CD5 B cells in development and disease.” Immunology Today.
Roederer, M., Ela, S. W., Staal, F. J. and Herzenberg, L. A. (1992). “N-acetylcysteine: a new approach to anti-HIV therapy.” AIDS Research & Human Retroviruses 8(2): 209-17.Abstract
Herzenberg, L. A. and Kantor, A. B. (1992). “Layered evolution in the immune system. A model for the ontogeny and development of multiple lymphocyte lineages.” Annals of the New York Academy of Sciences 651: 1-9.
Bhat, N. M., Kantor, A. B., Bieber, M. M., Stall, A. M., Herzenberg, L. A. and Teng, N. N. (1992). “The ontogeny and functional characteristics of human B-1 (CD5+ B) cells.” International Immunology 4(2): 243-52.Abstract
Kroese, F. G. M., Kantor, A. B. and Herzenberg, L. A. (1994). The role of B-1 cells in mucosal immune responses. The role of B-1 cells in mucosal immune responses. P. L. M. D. Ogra, J. M. D. Mestecky, M. E. M. D. Lammet al. New York, Academic Press, Inc.: 217-222.
Kantor, A. B. (1991). “A new nomenclature for B cells.” Immunology Today 12: 388.
Kantor, A. B., Stall, A. M., Adams, S. and Herzenberg, L. A. (1992). “Adoptive transfer of murine B-cell lineages.” Annals of the New York Academy of Sciences 651: 168-9.
Stall, A. M., Adams, S., Herzenberg, L. A. and Kantor, A. B. (1992). “Characteristics and development of the murine B-1b (Ly-1 B sister) cell population.” Annals of the New York Academy of Sciences 651: 33-43.Abstract
Staal, F. J., Ela, S. W., Roederer, M., Anderson, M. T. and Herzenberg, L. A. (1992). “Glutathione deficiency and human immunodeficiency virus infection [see comments].” Lancet 339(8798): 909-12.
Roederer, M., Staal, F. J., Ela, S. W. and Herzenberg, L. A. (1993). “N-acetylcysteine: potential for AIDS therapy.” Pharmacology 46(3): 121-9.Abstract
Kroese, F. G. M., Ammerlaan, Willem A.M., Kantor, Aaron B. (1993). “Evidence that intentinal IgA plasma cells in µ,k transgenic mice are derived from B-1 (Ly-1 B) cells.” International Immunology 5: 1317-1327.
Lalor, P., Bucci, C., Fornaro, M., Rattazzi, M. C., Nakauchi, H., Herzenberg, L. A. and Alberti, S. (1992). “Molecular cloning, reconstruction and expression of the gene encoding the alpha-chain of the bovine CD8--definition of three peptide regions conserved across species.” Immunology 76(1): 95-102.Abstract
Staal, F. J., Roederer, M., Raju, P. A., Anderson, M. T., Ela, S. W. and Herzenberg, L. A. (1993). “Antioxidants inhibit stimulation of HIV transcription.” AIDS Research & Human Retroviruses 9(4): 299-306.Abstract
Lam, K. P., Herzenberg, L. A. and Stall, A. M. (1993). “A high frequency of hybridomas from M54 mu heavy chain transgenic mice initially co-express transgenic and rearranged endogenous mu genes.” International Immunology 5(9): 1011-22.Abstract
Wells, S. M., Kantor, Aaron B., Stall, Alan M. (1994). “CD43 (S7) expression identified peripheral B cell subsets.” International Immunology 153: 5503-5515.
Kantor, A. B. and Herzenberg, L. A. (1993). “Origin of murine B cell lineages.” Annual Review of Immunology 11: 501-38.Abstract
Herzenberg, L. A. and Kantor, A. B. (1993). “B-cell lineages exist in the mouse.” Immunology Today 14(2): 79-83; discussion 88-90.
Russo-Marie, F., Roederer, M., Sager, B., Herzenberg, L. A. and Kaiser, D. (1993). “Beta-galactosidase activity in single differentiating bacterial cells.” Proceedings of the National Academy of Sciences of the United States of America 90(17): 8194-8.Abstract
Schildbach, J. F., Shaw, S. Y., Bruccoleri, R. E., Haber, E., Herzenberg, L. A., Jager, G. C., Jeffrey, P. D., Panka, D. J., Parks, D. R., Near, R. I. and et al. (1994). “Contribution of a single heavy chain residue to specificity of an anti- digoxin monoclonal antibody.” Protein Sci 3(5): 737-49.Abstract
Roederer, M., Staal, F. J., Anderson, M., Rabin, R., Raju, P. A. and Herzenberg, L. A. (1993). “Disregulation of leukocyte glutathione in AIDS.” Annals of the New York Academy of Sciences 677: 113-25.
LAH #376-01
Herzenberg, L. A. (1993). Introduction. Flow Cytometry and Cell Sorting: A Laboratory Manual. A. Radbruch. Heidelberg, Springer-Verlag: 1-3.
Staal, F. J. T., Roederer, M., Anderson, M.T., Herzenberg, L.A. (1993). Redox regulation of NF-kappaB activation and therefore of HIV expression. Molecular Basis of Immune Responses. Proceedings of the First Green Cross International Symposium, Tokyo, November 28-29, 1992. H. Saito, H. Nariuchi, N. Okadaet al. Tokyo, The Naito Foundation for Medical Research through Academcic Press/Harcourt Brace Jovanovich Japan, Inc.: 89-102.
Lam, K., Stall, A. M., Kantor, A. B. and Herzenberg, L. A. (1993). Origins of B cell lineages: Aspects of the difference between B-1 and conventional B cells in M54 µ transgenic mice. Molecular Basis of Immune Responses (Proceedings of the First Green Cross International Symposium, Tokyo, Nov. 28-9, 1992). H. Saito, H. Nariuchi, N. Okadaet al. Tokyo, Japan, The Naito Foundation for Medical Research through Academic Press/Harcourt Brace Jovanovich Japan, Inc.: 39-48.
Matsushima, T. (1993). Constructing a distributed object-oriented system with logical constraints for fluorescence-activated cell sorting. Proceedings of the "1st International Conference on Intelligent Systems for Molecular Biology" (July 6-9, 1993, Washington, D.C.). Washington, D.C., AAAI Press: 266-274.
Staal, F. J., Anderson, M. T., Staal, G. E., Herzenberg, L. A. and Gitler, C. (1994). “Redox regulation of signal transduction: tyrosine phosphorylation and calcium influx.” Proceedings of the National Academy of Sciences of the United States of America 91(9): 3619-22.Abstract
Anderson, M. T., Staal, F. J., Gitler, C. and Herzenberg, L. A. (1994). “Separation of oxidant-initiated and redox-regulated steps in the NF-kappa B signal transduction pathway.” Proceedings of the National Academy of Sciences of the United States of America 91(24): 11527-31.Abstract
Raju, P. A., Herzenberg, L. A. and Roederer, M. (1994). “Glutathione precursor and antioxidant activities of N-acetylcysteine and oxothiazolidine carboxylate compared in in vitro studies of HIV replication.” AIDS Research & Human Retroviruses 10(8): 961-7.Abstract
Bhat, N. M., Mithal, A., Bieber, M. M., Herzenberg, L. A. and Teng, N. N. (1995). “Human CD5+ B lymphocytes (B-1 cells) decrease in peripheral blood during pregnancy.” Journal of Reproductive Immunology 28(1): 53-60.Abstract
Montano, M. A., Kripke, K., Norina, C. D., Achacoso, P., Herzenberg, L. A., Roy, A. L. and Nolan, G. P. (1996). “NF-kappa B homodimer binding within the HIV-1 initiator region and interactions with TFII-I.” Proceedings of the National Academy of Sciences of the United States of America 93(22): 12376-81.Abstract
Rabin, R. L., Roederer, M., Maldonado, Y., Petru, A. and Herzenberg, L. A. (1995). “Altered representation of naive and memory CD8 T cell subsets in HIV-infected children.” Journal of Clinical Investigation 95(5): 2054-60.Abstract
Serafini, A. T., Lewis, R. S., Clipstone, N. A., Bram, R. J., Fanger, C., Fiering, S., Herzenberg, L. A. and Crabtree, G. R. (1995). “Isolation of mutant T lymphocytes with defects in capacitative calcium entry.” Immunity 3(2): 239-50.Abstract
Kroese, F. G. M., J.J. Cebra, M.J.F. van der Cammen, A.B. Kantor, N.A. Bos (1995). Contributuion of B-1 Cells to Intestinal IgA Production in the Mouse., Academic Press, Inc.
Bigos, M., Parks, D. R., Moore, W. A. and Herzenberg, L. A. (1994). “Pattern sorting: a computer-controlled multidimensional sorting method using k-d trees.” Cytometry 16(4): 357-63.Abstract
Staal, F. J., Anderson, M. T. and Herzenberg, L. A. (1995). “Redox regulation of activation of NF-kappa B transcription factor complex: effects of N-acetylcysteine.” Methods in Enzymology 252: 168-74.
Kroese, F. G., Ammerlaan, W. A., Deenen, G. J., Adams, S., Herzenberg, L. A. and Kantor, A. B. (1995). “A dual origin for IgA plasma cells in the murine small intestine.” Advances in Experimental Medicine & Biology 371A: 435-40.
Kroese, F. G. M., N.K. de Boer, T. de Boer, P. Nieuwenhuis, A.B. Kantor, G.J. Deenen (1995). Indentification and Linetics of Two Recently Bone Marrow-Derived B Cell Populations in Peripheral Lymphoid Tissues. Cellular Immunology, Academic Press. 162: 185-193.
Peterson, J. D., Herzenberg, L. A., Vasquez, K. and Waltenbaugh, C. (1998). “Glutathione levels in antigen-presenting cells modulate Th1 versus Th2 response patterns.” Proceedings of the National Academy of Sciences of the United States of America 95(6): 3071-6.Abstract
Alberti, S., Nutini, M. and Herzenberg, L. A. (1994). “DNA methylation prevents the amplification of TROP1, a tumor-associated cell surface antigen gene.” Proceedings of the National Academy of Sciences of the United States of America 91(13): 5833-7.Abstract
Wells, S. M., Stall, Alan M., Kantor, Aaron B., Herzenberg, Leonore A. (1995). Development of B Cell Subsets. Immunoglobulin Genes. T. Honjo and F. Alt. London, Academic Press Limited. Part I: B Cells, Chapter 4: 83-101.Abstract
The enormous diversity of antibody reactivities required to protect against the wide variety of antigens that an animal may encounter throughout life is generated by a complex differentiation process consisting of two basic stages. The variable region, which defines the antigen-combining site of the unique immunoglobulin molecule that each B cell produces, is initially determined by the developmentally-controlled juxtaposition of VH, D and JH elements of the heavy chain and VL and JL elements of the light chain. Variable-region rearrangement occurs during the early stages of a cell differentiation in the bone marrow and fetal liver. The first constant region domains (µ and ð) used in B cell development are determined by differential processing of the RNA transcripts. Later, as mature B cells in the periphery encounter specific antigens, the antibody molecules they produce may be further diversified by mechanisms that enable 'switching' of constant region (isotype) concomitant with somatic mutation of variable-region genes. This latter process, which can ultimately yield antibodies iwth higher affinities for the antigen, matures from a series of cell clones producing distinct but related immunoglobulin (Ig) molecules that often have markedly different binding affinities for the antigen.
The differentiation stages through which B cells and their progenitors pass on their way to becoming AFC initially appeared to follow a single developmental pathway. However, phenotypic and cell transfer studies begun in the early 1980s identified at least two independent B cell development pathways (lineages) whose progenitors are now distinguishable at an early developmental stage (pro-B cell), when the first Ig (D-JH) rearrangement begins (Hayakawa et al., 1984, 1985). T cells have now also been shown to differentiate along similarly separable developmental lineages, suggesting that the commitment to give rise to particular T and B lineages reflects the activity of distinctive lymphoid stem cells (Ikuta et al., 1990).
In this chapter, we focus primarily on the features that distinguish the developmental pathways of the two most well-established B cell lineages, commonly referred to as the B-1a (earlier designated Ly-1 or D5+ B) and conventional (or B-2) lineages (Kantor et al., 1991). We begin with a relatively brief description of the phenotypic and funcitonal characteristics of the developmental stages of B cells. Then, starting with the most mature B cells and working back towards the early stages of B cell development, we detail the differences between the cells in each lineage at these various stages of development. Finally, we discuss the features that potentially distinguish additional B cell developmental lineages and discuss the evolutionary and functional consequences of these multiple layers of functionally related cells in the immune system.
Kantor, A. B., Stall, A. M., Adams, S., Watanabe, K. and Herzenberg, L. A. (1995). “De novo development and self-replenishment of B cells.” International Immunology 7(1): 55-68.Abstract
Nakamura, H., De Rosa, S., Roederer, M., Anderson, M. T., Dubs, J. G., Yodoi, J., Holmgren, A. and Herzenberg, L. A. (1996). “Elevation of plasma thioredoxin levels in HIV-infected individuals.” International Immunology 8(4): 603-11.Abstract
Roederer, M., Dubs, J. G., Anderson, M. T., Raju, P. A. and Herzenberg, L. A. (1995). “CD8 naive T cell counts decrease progressively in HIV-infected adults.” Journal of Clinical Investigation 95(5): 2061-6.Abstract
Herzenberg, L. A. (1994). Forward to the Third Edition of Practicle Flow Cytometry. Practical Flow Cytometry. H. M. Shapiro, Wiley-Liss.
Roederer, M., Bigos, M., Nozaki, T., Stovel, R. T., Parks, D. R. and Herzenberg, L. A. (1995). “Heterogeneous calcium flux in peripheral T cell subsets revealed by five-color flow cytometry using log-ratio circuitry.” Cytometry 21(2): 187-96.Abstract
Katsikis, P. D., Wunderlich, E. S., Smith, C. A. and Herzenberg, L. A. (1995). “Fas antigen stimulation induces marked apoptosis of T lymphocytes in human immunodeficiency virus-infected individuals.” Journal of Experimental Medicine 181(6): 2029-36.Abstract
Kantor, A. B., Merrill, C. E., MacKenzie, J. D., Herzenberg, L. A. and Hillson, J. L. (1995). “Development of the antibody repertoire as revealed by single-cell PCR of FACS-sorted B-cell subsets.” Annals of the New York Academy of Sciences 764: 224-7.
Roederer, M. and Herzenberg, L. A. (1996). “Changes in antigen densities on leukocyte subsets correlate with progression of HIV disease.” International Immunology 8(1): 1-11.Abstract
Tokuhisa, T., Herzenberg, Leonore A. (1996). Epitope-Specific Regulation of Antibody Responses. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science.
Kantor, A. B., Merrill, C.E.Merrill, J.L. Hillson (1996). Construction of cDNA from Single Unstimulated Mouse B Lymphocytes: Method and Application to the Study of Expressed Antibody Repertoires in FACS-Sorted Murine B Cell Subsets. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science: 13.1-13.6.
Gerstein, R. M. (1996). The Generation of Junctional Diversity by V(D)J Recombination. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science: 14.1-14.9.
Parks, D. R., Kantor, A.B., Roederer, M. (1996). Overview: Flow Cytometry and FACS. The Handbook of Experiemental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science: 46.1-46.2.
Kantor, A. B., M. Roederer (1996). FACS Analysis of Leukocytes. The handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science: 43.1-49.13.
Tung, J. W., Kantor, A. B. and L.A., H. (1997). Quantitative competitve PCR. The Handbook of Experimental Immunology. Boston, Balckwell Science. 2 Cell Surface and Messenger Molecules of the Immune System: 59.1-59.6.
Anderson, M. T., M. Roederer., I. Tjioe., L.A. Herzenberg and L.A. Herzeneberg. (1997). Glutathione Measurement of the Flow Cytometer. Handbook of Experimental Immunology. W. Herzenberg, Blackwell, & Herzenberg. Boston, Blackwell Science. 2, Cell Surface and Messenger Molecules of the Immune System, Section of Flow Cytometry(FACS) Chapter 54: 54.1-54.9.
Katsikis, P. D., E.S. Wunderlich, M. Amano (1996). Apoptosis and the Immune System: Flow Cytometry Techniques for Measurement of Apoptosis. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science: 55.1-55.5.
Khan, W. N., Alt, F. W., Gerstein, R. M., Malynn, B. A., Larsson, I., Rathbun, G., Davidson, L., Muller, S., Kantor, A. B., Herzenberg, L. A. and et al. (1995). “Defective B cell development and function in Btk-deficient mice.” Immunity 3(3): 283-99.Abstract
Parks, D. R. (1996). Flow Cytometry Instrumentation and Measurements. The handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science.
Parks, D. R., M. Bigos (1996). Collection, Display and Analysis of Flow Cytometry Data. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell Science: 50.1-50.11.
Stall, A. M. (1996). Mouse Immunoglobulin Allotypes. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell: 27.1-27.16.
Lorincz, M., Roederer, M., Diwu, Z., Herzenberg, L. A. and Nolan, G. P. (1996). “Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli beta-glucuronidase.” Cytometry 24(4): 321-9.Abstract
Murphy, D. B. and Herzenberg, L. A. (1997). Overview: Specialized Mouse Strains and Study of Gene Expression and Function. Handbook of Experimental Immunology 5th Edition Section 25, Chapter 149. W. Herzenberg, Blackwell, Herzenberg. Boston, Blackwell Sciences: 149.1-149.1.
Kantor, A. B., Merrill, C. E., Herzenberg, L. A. and Hillson, J. L. (1997). “An unbiased analysis of V(H)-D-J(H) sequences from B-1a, B-1b, and conventional B cells.” Journal of Immunology 158(3): 1175-86.Abstract
Kerr, W. G., Heller, M. and Herzenberg, L. A. (1996). “Analysis of lipopolysaccharide-response genes in B-lineage cells demonstrates that they can have differentiation stage-restricted expression and contain SH2 domains.” Proceedings of the National Academy of Sciences of the United States of America 93(9): 3947-52.Abstract
Katsikis, P. D., Garcia-Ojeda, M. E., Wunderlich, E. S., Smith, C. A., Yagita, H., Okumura, K., Kayagaki, N., Alderson, M. and Herzenberg, L. A. (1996). “Activation-induced peripheral blood T cell apoptosis is Fas independent in HIV-infected individuals.” International Immunology 8(8): 1311-7.Abstract
Anderson, M. T., Tjioe, I. M., Lorincz, M. C., Parks, D. R., Herzenberg, L. A. and Nolan, G. P. (1996). “Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins.” Proceedings of the National Academy of Sciences of the United States of America 93(16): 8508-11.Abstract
Weirich, E., Rabin, R. L., Maldonado, Y., Benitz, W., Modler, S. and Herzenberg, L. A. (1998). “Neutrophil CD11b expression as a diagnostic marker for early-onset neonatal infection.” Journal of Pediatrics 132(3 Pt 1): 445-51.Abstract
Roederer, M., Kantor, A. B., Parks, D. R. and Herzenberg, L. A. (1996). “Cy7PE and Cy7APC: bright new probes for immunofluorescence.” Cytometry 24(3): 191-7.Abstract
Roederer, M., Landay, A. (1996). Flow Cytometric Evaluation in AIDS. The Handbook of Experimental Immunology. L. Herzenberg, L. Herzenberg, C. Blackwell and D. Weir. Boston, Blackwell: 182.1-182.12.
Seidl, K. J., MacKenzie, J. D., Wang, D., Kantor, A. B., Kabat, E. A. and Herzenberg, L. A. (1997). “Frequent occurrence of identical heavy and light chain Ig rearrangements.” International Immunology 9(5): 689-702.Abstract
Herzenberg, L. A., De Rosa, S. C., Dubs, J. G., Roederer, M., Anderson, M. T., Ela, S. W. and Deresinski, S. C. (1997). “Glutathione deficiency is associated with impaired survival in HIV disease.” Proceedings of the National Academy of Sciences of the United States of America 94(5): 1967-72.Abstract
Roederer, M., Parks D.R., Herzenberg L.A., Herzenberg L.A. (1996). Flow Cytometry. Encyclopedia of Immunology. R. a. Delves. London, Academic. 2nd Edition: 932-943.
Herzenberg, L. A., De Rosa S., Herzenberg L.A. (1998). Low Glutathione Levels in CD4 T Cells Predict Poor Survival in AIDS; N-Acetyclsteine May Improve Survival. Oxidative Stress in Cancer, AIDS and Neuodegerative Diseases. O. R. Monatagnier L, Pasquier C. Stanford, CA, Marcel Dekker, Inc. 1: 379-387.
Seidl, K. J., MacKenzie, J. D., Wang, D., Kantor, A. B., Kabat, E. A. and Herzenberg, L. A. (1997). “Recurrent identical rearrangement and repeated expression of identical heavy and light chains in single anti-phosphatidylcholine B cells.” Annals of the New York Academy of Sciences 815: 484-8.
LAH #430
de Waard, R., Dammers, P. M., Tung, J. W., Kantor, A. B., Wilshire, J. A., Bos, N. A., Herzenberg, L. A. and Kroese, F. G. (1998). “Presence of germline and full-length IgA RNA transcripts among peritoneal B-1 cells.” Developmental Immunology 6(1-2): 81-7.Abstract
Lorincz, M., Herzenberg, L. A., Diwu, Z., Barranger, J. A. and Kerr, W. G. (1997). “Detection and isolation of gene-corrected cells in Gaucher disease via a fluorescence-activated cell sorter assay for lysosomal glucocerebrosidase activity.” Blood 89(9): 3412-20.Abstract
Katsikis, P. D., Garcia-Ojeda, M. E., Torres-Roca, J. F., Greenwald, D. R. and Herzenberg, L. A. (1997). “HIV type 1 Tat protein enhances activation-but not Fas (CD95)-induced peripheral blood T cell apoptosis in healthy individuals.” International Immunology 9(6): 835-41.Abstract
Zambrowicz, B. P., Imamoto, A., Fiering, S., Herzenberg, L. A., Kerr, W. G. and Soriano, P. (1997). “Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells.” Proceedings of the National Academy of Sciences of the United States of America 94(8): 3789-94.Abstract
Amano, M., Baumgarth, N., Dick, M. D., Brossay, L., Kronenberg, M., Herzenberg, L. A. and Strober, S. (1998). “CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: beta 2-microglobulin-dependent and independent forms.” Journal of Immunology 161(4): 1710-7.Abstract
Roederer, M., DeRosa, S., Herzenberg, L. A. and Herzenberg, L. A. (1996). Immunology of T cells in AIDS: Dynamics revealed by 8-color flow cytometry. Handbook of Immunology. New Yokr, Marcel Deller, Inc.: 209-220.
Roederer, M., Raju, P. A., Mitra, D. K. and Herzenberg, L. A. (1997). “HIV does not replicate in naive CD4 T cells stimulated with CD3/CD28.” Journal of Clinical Investigation 99(7): 1555-64.Abstract
LAH #437
Roederer, M., Herzenberg L.A. (1997). Flow Cytometry: A Powerful Tool for Molecular Biology. The Encyclopedia of Molecular Biology 1st Edition. Creighton. New York, John Wiley and Sons: IN PRESS.
Watanabe, N., De Rosa, S. C., Cmelak, A., Hoppe, R., Herzenberg, L. A. and Roederer, M. (1997). “Long-term depletion of naive T cells in patients treated for Hodgkin's disease.” Blood 90(9): 3662-72.Abstract
Roederer, M., De Rosa, S., Gerstein, R., Anderson, M., Bigos, M., Stovel, R., Nozaki, T., Parks, D. and Herzenberg, L. (1997). “8 color, 10-parameter flow cytometry to elucidate complex leukocyte heterogeneity.” Cytometry 29(4): 328-39.Abstract
Roederer, M., De Rosa, S. C., Watanabe, N. and Herzenberg, L. A. (1997). “Dynamics of fine T-cell subsets during HIV disease and after thymic ablation by mediastinal irradiation.” Seminars in Immunology 9(6): 389-96.Abstract
Katsikis, P. D., Garcia-Ojeda, M. E., Torres-Roca, J. F., Tijoe, I. M., Smith, C. A. and Herzenberg, L. A. (1997). “Interleukin-1 b converting enzyme-like protease involvement in Fas-induced and activation-induced peripheral blood T cell apoptosis in HIV infection. TNF-related apoptosis-inducing ligand can mediate activation-induced T cell death in HIV infection.” Journal of Experimental Medicine 186(8): 1365-72.Abstract
Lorincz, M. C., Parente, M. K., Roederer, M., Nolan, G. P., Diwu, Z., Martin, D. I., Herzenberg, L. A. and Wolfe, J. H. (1999). “Single cell analysis and selection of living retrovirus vector-corrected mucopolysaccharidosis VII cells using a fluorescence-activated cell sorting-based assay for mammalian beta-glucuronidase enzymatic activity.” Journal of Biological Chemistry 274(2): 657-65.Abstract
Anderson, M. T., Baumgarth, N., Haugland, R. P., Gerstein, R. M., Tjioe, T. and Herzenberg, L. A. (1998). “Pairs of violet-light-excited fluorochromes for flow cytometric analysis.” Cytometry 33(4): 435-44.Abstract
Torres-Roca, J., Greenwald, D., Brown, J., Weissman, I., Herzenberg, L., Herzenberg, L. and Katsikis, P. (1997). “Very-low oxygen conditions distinguish between oxygen-dependent and independent apoptosis.” J. of Allergy and Clin. Immun. 99(1/pt.2): 1276.
Galbraith, D. W., Anderson, M. T. and Herzenberg, L. A. (1999). “Flow cytometric analysis and FACS sorting of cells based on GFP accumulation.” Methods in Cell Biology 58: 315-41.
Baumgarth, N., Herman, O. C., Jager, G. C., Brown, L. and Herzenberg, L. A. (1999). “Innate and acquired humoral immunities to influenza virus are mediated by distinct arms of the immune system.” Proceedings of the National Academy of Sciences of the United States of America 96(5): 2250-5.Abstract
LAH #448
De Rosa, S. C., Zaretsky, M. D., Dubs, J. G., Roederer, M., Anderson, M., Green, A., Dipendra Mitra, M., Watanabe, N., Nakamura, H., Tjioe, I., Deresinski, S. C., Moore, W. A., Ela, S. W., Parks, D., Herzenberg, L. A. and Herzenberg, L. A. (2000). "N-acetylcysteine (NAC) replenishes glutathione in HIV infection." European Journal of Clinical Investigation: 30(10):915-929.Abstract
Design Oral NAC administration in a randomized, 8-week double-blind, placebo-controlled trial followed by optional open-label drug for up to 24 weeks. Subjects HIV-infected, low GSH, CD4 T cells < 500 mL -1 , no active opportunistic infections or other debilitation; h=81. Study conducted prior to introduction of protease inhibitors.Results Whole blood GSH levels in NAC arm subjects significantly increased from 0.88 mM to 0.98 mM, bringing GSH levels in NAC-treated subjects to 89% of uninfected controls (P =0.03). Baseline GSH levels in the placebo group (0.91) remained essentially the same during the 8 week placebo-controlled trial. T cell GSH, adjusted for CD4 T cell count and b2-microglobulin levels, also increased in the NAC-treated subjects (P ‹0.04). Adverse effects were minimal and not significantly associated with NAC ingestion. Conclusion. NAC treatment for 8 weeks safely replenishes whole blood GSH and T cell GSH in HIV-infected individuals. Thus, NAC offers useful adjunct therapy to increase protection against oxidative stress, improve immune system function and increase detoxification of acetaminophen and other drugs. These findings suggest that NAC therapy could be valuable in other clinical situations in which GSH deficiency or oxidative stress plays a role in disease pathology, e.g. rheumatoid arthritis, Parkinson's disease, hepatitis, liver cirrhosis, septic shock and diabetes.Keywords glutathione, GSH, GSH deficiency, HIV, N-acetylcysteine, NAC Review of article LAH #448.
Seidl, K. J., Wilshire, J. A., MacKenzie, J. D., Kantor, A. B. and Herzenberg, L. A. (1999). “Predominant VH genes expressed in innate antibodies are associated with distinctive antigen-binding sites.” Proceedings of the National Academy of Sciences of the United States of America 96(5): 2262-7.Abstract
LAH #451
Tung, J. W., Kunnavatana, S. S. and Herzenberg, L. A. (2001). “The regulation of CD5 expression in murine T cells.” BMC Mol Biol 2(1): 5.Abstract
RESULTS: We further characterize the CD5 promoter and identify minimal and regulatory regions on the CD5 promoter. Using a luciferase reporter system, we show that a 43 bp region on the CD5 promoter regulates CD5 expression in resting mouse thymoma EL4 T cells and that an Ets binding site within the 43 bp region mediates the CD5 expression. In addition, we show that Ets-1, a member of the Ets family of transcription factors, recognizes the Ets binding site in the electrophoretic mobility shift assay (EMSA). This Ets binding site is directly responsible for the increase in reporter activity when co- transfected with increasing amounts of Ets-1 expression plasmid.We also identify two additional evolutionarily-conserved regions in the CD5 promoter (CD5X and CD5Y) and demonstrate the respective roles of the each region in the regulation of CD5 transcription.
CONCLUSION: Our studies define a minimal and regulatory promoter for CD5 and show that the CD5 expression level in T cells is at least partially dependent on the level of Ets-1 protein. Based on the findings in this report, we propose a model of CD5 transcriptional.
Anderson, M. T., Trudell, J. R., Voehringer, D. W., Tjioe, I. M. and Herzenberg, L. A. (1999). “An improved monobromobimane assay for glutathione utilizing tris- (2-carboxyethyl)phosphine as the reductant.” Analytical Biochemistry 272(1): 107-9.
Bigos, M., Baumgarth, N., Jager, G. C., Herman, O. C., Nozaki, T., Stovel, R. T., Parks, D. R. and Herzenberg, L. A. (1999). “Nine color eleven parameter immunophenotyping using three laser flow cytometry.” Cytometry 36(1): 36-45.Abstract
Nakamura, H., De Rosa, S. C., Roedererd, M., Yodoi, J., Holmgren, A., Herzenberg, L. A. and Herzenberg, L. A. (2001). “Chronic elevation of plasma thioredoxin: inhibition of chemotaxis and curtailment of life expectancy in AIDS.” Proceedings of the National Academy of Sciences 98(5): 26885-2693.Abstract
Roederer, M., L.A. Herzenberg (1999). Flow Cytometry. Encyclopedia of Molecular Biology. Creighton. New York, NY, John Wiley & Sons, Inc.: 1-4.
Bertini, R., Howard, O. M., Dong, H. F., Oppenheim, J. J., Bizzarri, C., Sergi, R., Caselli, G., Pagliei, S., Romines, B., Wilshire, J. A., Mengozzi, M., Nakamura, H., Yodoi, J., Pekkari, K., Gurunath, R., Holmgren, A., Herzenberg, L. A. and Ghezzi, P. (1999). “Thioredoxin, a redox enzyme released in infection and inflammation, is a unique chemoattractant for neutrophils, monocytes, and T cells.” Journal of Experimental Medicine 189(11): 1783-9.Abstract
Voehringer, D. W., Hirschberg, D. L., Xiao, J., Lu, Q., Roederer, M., Lock, C. B., Herzenberg, L. A. and Steinman, L. (2000). “Gene microarray identification of redox and mitochondrial elements that control resistance or sensitivity to apoptosis.” Proc Natl Acad Sci U S A 97(6): 2680-2685.Abstract
Baumgarth, N., Jager, G. C., Herman, O. C. and Herzenberg, L. A. (2000). "CD4+ T cells derived from B cell-deficient mice inhibit the establishment of peripheral B cell pools." Proc Natl Acad Sci U S A 97(9): 4766-4771.Abstract
Andrus, J. P., De Rosa, S. C., Tjioe, I. M., Frankel, L. R., Roederer, M., Herzenberg, L. A. and Herzenberg, L. A. (1999). “Depressed Lymphocyte glutathione levels in patients with septic shock.” Journal of Investigative Medicine 47(2 - Feb. 99): A155.
Galbraith, D. W., Herzenberg, L. A. and Anderson, M. T. (1999). Flow Cytometric Analysis of Transgene Expression in Higher Plants: Green Fluorescent Protein. Methods in Enzymology. Conn. New York, John Wiley and Sons. 302: 296-315.
Herzenberg, L. A. (1999). Introduction to the Second Edition. Flow Cytometry and Cell Sorting. Radbruch. Heidelberg, Springer-Verlag: i.
Lee, P. P., Yee, C., Savage, P. A., Fong, L., Brockstedt, D., Weber, J. S., Johnson, D., Swetter, S., Thompson, J., Greenberg, P. D., Roederer, M. and Davis, M. M. (1999). “Characterization of Circulating T cell specific for tumor-associated antigens in melanoma patients.” Nat. Med. 5(6): 677-685.
Mitra, D. K., De Rosa, S. C., Luke, A., Balamurugan, A., Khaitan, B. K., Tung, J., Mehra, N. K., Terr, A. I., O'Garra, A., Herzenberg, L. A. and Roederer, M. (1999). “Differential representations of memory T cell subsets are characteristic of polarized immunity in leprosy and atopic diseases.” International Immunology 11(11): 1801-10.Abstract
Herzenberg, L. A., Moore, W. A. and De Rosa, S. C. (1999). “Estimation of missing values.” Lancet 354(9179): Correspondence.
LAH #466
Baumgarth, N., Herman, O. C., Jager, G. C., Brown, L. E., Herzenberg, L. A. and Chen, J. (2000). “B-1 and B-2 cell–derived immunoglobulin M antibodies are nonredundant components of the protective response to influenza virus infection.” Journal of Experimental Medicine, IN PRESS JULY17, 192(2).
LAH #467
Baumgarth, N., Chen, J., Herman, O., Jager, G. and Herzenberg, L. (1999). The Role of B-1 and B-2 Cells in Immune Protection from Influenza Virus Infection. 16th Workshop on the Mechanisms of B Cell Neoplasia 1999, Lister Hill Auditorium, National Library of Medicine, NIH, Bethesda, MD, Springer-Verlag.
LAH #468
Baumgarth, N. and Roederer, M. (2000). "A practical approach to multicolor flow cytometry for immunophenotyping." J Immunol Methods 243(1-2): 77-97..Abstract
LAH #469
Herzenberg, L., Baumgarth, N. and Wilshire, J. (1999). B-1 cell origins and VH repertoire determination. 16th Workshop on the Mechanisms of B Cell Neoplasia 1999, Lister Hill Auditorium, National Library of Medicine, NIH, Bethesda, MD, Springer-Verlag.
LAH #470
De Rosa, S., Herzenberg, L., Herzenberg, L. and Roederer, M. (2001). “11 color, 13 parameter Flow Cytometry: Identification of naive T cells by phenotype, function and T cell receptor.” Nature Medicine 7(2): 245-248.Abstract
LAH #471
Ghezzi, P., B. Romines, M. Fratelli, I. Eberini, E. Gianazza, S. Casagrande, T. Laragione, M. Mengozzi and L. A. Herzenberg (2002). "Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection." Mol Immunol 38(10): 773-80.Abstract
LAH #472
Herzenberg, L. A., S. C. De Rosa and L. A. Herzenberg (2000). "Monoclonal Antibodies and the FACS: complementary tools for immunobiology and medicine." Immunology Today 21(8): 383-390.Abstract
The Fluorescence Activated Cell Sorter (FACS) was born in our laboratory, ca 1968, after a long but natural labor. As an infant, it was cantankerous and slow and required intensive care and feeding. Nonetheless, it began to do productive work quite rapidly and, by 1969, we announced its birth in a publication demonstrating the automated separation of mammalian plasma cells as a function of intracellular fluorescence3. Over the next two years, we introduced improvements, including laser illumination (which replaced the earlier arc lamp) that made the nascent FACS sensitive enough to detect fluorescent-labeled antibodies bound to mammalian lymphoid cells. Thus, by 1971, we were able to count, sort and functionally characterize T and B cells tagged with fluorescent antibodies or antigens that bound to cell surface determinants4. By 1972, we began to use the FACS to answer immunologically relevant questions by labeling cells with fluorochrome-coupled reagents (conventional antibodies and protein antigens) and following the fate of sorted cells in adoptive recipients. The first of these studies showed that antigen-binding cells are the precursors of antibody producing cells2. Next, in a series of B cell commitment studies5-9, we and our collaborators defined relationships between surface Ig isotype and subsequent isotype production and, in studies documenting allelic (haplotype) exclusion, showed that surface Ig allotype in allotype heterozygotes reveals the allotype production potential (chromosomal commitment for all isotypes) of B cells and their progeny. Collectively, results from these studies set the stage for today's understanding of the mechanisms of allelic exclusion, Ig chromosome rearrangement and overall B cell development.The first FACS characterization of human T cells, B cells and other peripheral blood leukocytes were conducted during these early years10,11. In addition, substantial progress was made characterizing murine T cell surface markers and functions12-17. Thus, by the time commercial FACS instruments became available, the potential for using these instruments for immunological studies was well established.
LAH #473
Herzenberg, L. A. (2000). "B-1 Cells: the lineage question revisisted." Immunological Reviews 175: 9-21.
LAH #474
Andrus, J. P., L. A. Herzenberg and S. C. DeRosa (2001). "Effects of legislation restricting pack sizes of paracetamol on self poisoning. Paracetamol should be packaged with its antidote." Bmj 323(7313): 634.Abstract
LAH #475
Mengozzi, M., M. Malipatlolla, S. C. De Rosa, L. A. Herzenberg and M. Roederer (2001). "Naive CD4 T cells inhibit CD28-costimulated R5 HIV replication in memory CD4 T cells." Proc Natl Acad Sci U S A 98(20): 11644-9.Abstract
LAH #476
Perez, O. D., G. P. Nolan, D. Magda, R. A. Miller, L. A. Herzenberg and L. A. Herzenberg (2002). "Motexafin gadolinium (Gd-Tex) selectively induces apoptosis in HIV-1 infected CD4+ T helper cells." PNAS 99(4): 2270-2274.Abstract
LAH #477
Tjioe, I., T. Legerton, J. Wegstein, L. A. Herzenberg and M. Roederer (2001). "Phycoerythrin-allophycocyanin: A resonance energy transfer fluorochrome for immunofluorescence." Cytometry 44(1): 24-9.Abstract
Breithaupt, T. B., A. L. Shires, D. W. Voehringer and L. A. Herzenberg (2001). "Isoelectric focusing and enzyme overlay membrane analysis of caspase 3 activation." Anal Biochem 292(2): 313-6.
Torres-Roca JF, Tung JW, Greenwald DR, J. Martin Brown, Herzenberg LA, Herzenberg LA, et al. An Early Oxygen-Dependent Step Is Required for Dexamethasone-Induced Apoptosis of Immature Mouse Thymocytes1. The Journal of Immunology,. 2000(1465):4822-30.Abstract
LAH #480
Lu, L.-S., J. Tung, N. Baumgarth, O. Herman, M. Gleimer, L. A. Herzenberg and L. A. Herzenberg (2002). "Identification of a germ-line pro-B cell subset that distinguishes the fetal/neonatal from the adult B cell development pathway." PNAS: 052715399.Abstract
LAH #481
Salomon, A. R., D. W. Voehringer, L. A. Herzenberg and C. Khosla (2000). "Understanding and exploiting the mechanistic basis for selectivity of polyketide inhibitors of F0F1-ATPase." Proc Natl Acad Sci U S A 97(26): 14766-14771.Abstract
LAH #482
Torres-Roca, J. F., J. W. Tung, D. R. Greenwald, J. M. Brown, L. A. Herzenberg and P. D. Katsikis (2000). "An early oxygen-dependent step is required for dexamethasone-induced apoptosis of immature mouse thymocytes." J Immunol 165(9): 4822-30.Abstract
LAH #484
Roederer, M., A. Treister, W. A. Moore and L. A. Herzenberg (2001). "Probability binning comparison: A metric for quantitating univariate distribution differences." Cytometry 45(1): 37-46.Abstract
LAH #485
Roederer, M., W. A. Moore, A. Treister, R. R. Hardy and L. A. Herzenberg (2001). "Probability binning comparison: a metric for quantitating multivariate distribution differences." Cytometry 45(1): 47-55.Abstract
LAH #486
Salomon, A. R., D. W. Voehringer, L. A. Herzenberg and C. Khosla (2001). "Apoptolidin, a selective cytotoxic agent, is an inhibitor of F(0)F(1)- ATPase." Chem Biol 8(1): 71-80.Abstract
LAH #487
Napolitano, L. A., R. M. Grant, S. G. Deeks, D. Schmidt, S. C. De Rosa, L. A. Herzenberg, B. G. Herndier, J. Andersson and J. M. McCune (2001). "Increased production of IL-7 accompanies HIV-1-mediated T-cell depletion: implications for T-cell homeostasis." Nat Med 7(1): 73-79.Abstract
LAH #488
De Rosa, S. C., D. K. Mitra, N. Watanabe, L. A. Herzenberg and M. Roederer (2001). " delta1 and delta2 gammadelta T cells express distinct surface markers and might be developmentally distinct lineages." J Leukoc Biol 70(4): 518-26.Abstract
LAH #490
Herzenberg, L. A., D. Parks, B. Sahaf, O. Perez, M. Roederer and L. A. Herzenberg (2002). "The History and Future of the Fluorescence Activated Cell Sorter and Flow Cytometry: A View from Stanford." Clin Chem 48(10): 1819-1827.Abstract
LAH #491
Nakamura, H., L. A. Herzenberg, J. Bai, S. Araya, N. Kondo, Y. Nishinaka, L. A. Herzenberg and J. Yodoi (2001). "Circulating thioredoxin suppresses lipopolysaccharide-induced neutrophil chemotaxis." PNAS 98(26): 15143-15148.Abstract
LAH #492
Tsuji, R. F., M. Szczepanik, I. Kawikova, V. Paliwal, R. A. Campos, A. Itakura, M. Akahira-Azuma, N. Baumgarth, L. A. Herzenberg and P. W. Askenase (2002). "B Cell-dependent T Cell Responses: IgM Antibodies Are Required to Elicit Contact Sensitivity." J Exp Med 196(10): 1277-1290.Abstract
LAH #493
Borghesi, L., L. Y. Hsu, et al. (2004). "B Lineage-specific Regulation of V(D)J Recombinase Activity Is Established in Common Lymphoid Progenitors." J Exp Med 199(4): 491-502.Abstract
LAH #494
Sahaf, B., K. Heydari, et al. (2003). "Lymphocyte surface thiol levels." Proc Natl Acad Sci U S A 100(7): 4001-5.Abstract
LAH #495
Perez, O. D., D. Mitchell, et al. (2003). "Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1." Nat Immunol 4(11): 1083-92.Abstract
LAH #496
De Rosa, S. C., J. P. Andrus, et al. (2004). "Ontogeny of {gamma}{delta} T Cells in Humans." J Immunol 172(3): 1637-1645.Abstract
LAH #497
Tung, J. W., D. R. Parks, et al. (2004). Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods. Methods Mol Biol. 271: 37-58.Abstract
LAH #498
LAH #498-01 Supplemental Material
Tirouvanziam, R., C. J. Davidson, et al. (2004). "Fluorescence-activated cell sorting (FACS) of Drosophila hemocytes reveals important functional similarities to mammalian leukocytes." Proc Natl Acad Sci U S A 101(9): 2912-2917.Abstract
LAH #499
Tung, J. W., D. R. Parks, et al. (2004). "New approaches to fluorescence compensation and visualization of FACS data." Clin Immunol 110(3): 277-83.Abstract
LAH #500
Herzenberg, L. A. and L. A. Herzenberg (2004). "Genetics, FACS, Immunology, and Redox: A Tale of Two Lives Intertwined." Annual Review of Immunology 0(0).Abstract
LAH #501
LAH #501-1 Supporting Material
LAH #501-2 Supporting Material
Yang, Y., C. H. Contag, et al. (2004). "The E47 transcription factor negatively regulates CD5 expression during thymocyte development." Proc Natl Acad Sci U S A 101(11): 3898-902.Abstract
LAH #502
LAH #502-1 Related Material
Herzenberg, L. A. (2004). "FACS innovation: a view from Stanford." Clin Invest Med 27(5): 240-52.
LAH #503
Davidson, C., R. Tirouvanziam, L. Herzenberg and J. Lipsick (2004). "Functional Evolution of the Vertebrate Myb Gene Family: B-Myb, but neither A-Myb nor c-Myb, complements Drosophila Myb in Hemocytes." Genetics.Abstract
LAH #504
Cao, Y. A., M. H. Bachmann, et al. (2005). "Molecular imaging using labeled donor tissues reveals patterns of engraftment, rejection, and survival in transplantation." Transplantation 80(1): 134-9.Abstract
LAH #505
Gernez, Y., Herzenberg, L. A., Herzenberg, L. A. and Tirouvanziam, R. (2007). "[Phospho-Facs: A Powerful Tool for Exploring Intracellular Transduction Cascades.]." Rev Mal Respir 24(8): 955-64.Abstract
STATE OF THE ART: Recently, the use of fluorescent antibodies specific for phosphorylated epitopes - or "phospho-epitopes" - within proteins of interest has further extended the range of FACS analyses. This new application, dubbed "phospho-FACS", has quickly become a tool of choice for delineating intracellular phosphorylation cascades.PERSPECTIVES: In both basic research and clinical research, the application of phospho-FACS to cellular subsets from blood or the periphery, whether frequent or rare, enables the discovery of pathological biomarkers and therapeutic innovation.CONCLUSIONS: Thanks to its rapid implementation and its ability to generate single-cell data, the phospho-FACS technique features numerous advantages compared to preexisting analytical methods for intracellular phosphorylation cascades.
LAH #506
Baumgarth, N., J. W. Tung and L. A. Herzenberg (2005). "Inherent specificities in natural antibodies: a key to immune defense against pathogen invasion." Springer Semin Immunopathol.Abstract
LAH #507
Atkuri, K. R., L. A. Herzenberg and L. A. Herzenberg (2005). "Culturing at atmospheric oxygen levels impacts lymphocyte function." Proc Natl Acad Sci U S A 102(10): 3756-9.Abstract
LAH #508
Sahaf, B., K. Heydari, L. A. Herzenberg and H. L. A. (2005). "The extracellular microenvironment plays a key role in regulating the redox status of cell surface proteins in HIV-infected subjects." Archives of Biochemistry and Biophysics 434(1): 26-32.Abstract
LAH #509
Gernez, Y., Tirouvanziam, R., Nguyen, K. D., Herzenberg, L. A., Krensky, A. M. and Nadeau, K. C. (2007). "Altered Phosphorylated Signal Transducer and Activator of Transcription Profile of Cd4(+)Cd161(+) T Cells in Asthma: Modulation by Allergic Status and Oral Corticosteroids." J Allergy Clin Immunol.Abstract
OBJECTIVE: We tested whether asthmatic patients showed any change in cytokine-dependent signal transducer and activator of transcription (STAT) levels, focusing on the central/effector-memory CD4(+)CD161(+) subset, which represents 15% to 25% of circulating T cells. METHODS: We quantified intracellular levels of active phosphorylated STAT (phospho-STAT) 1, 3, 5, and 6 by means of flow cytometry, without any activation or expansion. RESULTS: Baseline phospho-STAT1 and phospho-STAT6 levels were increased in CD4(+)CD161(+) T cells from asthmatic patients compared with those from healthy control subjects (by 10- and 8-fold, respectively). This asthma-associated alteration was both subset specific because no change was seen in CD4(+)CD161(-)CD25(+) (regulatory T cells) and CD4(+)CD161(-)CD25(-) subsets and isoform specific because phospho-STAT5 and phospho-STAT3 levels were unchanged. Among asthmatic patients, phospho-STAT1 and phospho-STAT6 levels correlated negatively with each other, suggesting antagonistic regulation. Oral corticosteroid (OCS) treatment significantly decreased phospho-STAT6 and IL-4 levels but not phospho-STAT1 levels. Disease parameters showing significant correlations with phospho-STAT1, phospho-STAT6, or both included age at onset, plasma IgE levels, and levels of the T(H)2 cytokines IL-4 and IL-10 and the T(H)1 cytokine IL-2. Overall, combined phospho-STAT1 and phospho-STAT6 measurements showed excellent predictive value for identifying (1) asthmatic patients versus healthy control subjects, (2) allergic versus nonallergic asthmatic patients, and (3) asthmatic patients taking versus those not taking OCSs.
CONCLUSION: Baseline changes in phospho-STAT1 and phospho-STAT6 levels in blood CD4(+)CD161(+) T cells identify asthmatic patients and mirror their allergic status and response to OCSs.
CLINICAL IMPLICATIONS: These results confirm the pathologic importance of activated STAT1 and STAT6 in asthma and suggest their potential use as clinical biomarkers.
LAH #510
Ray, S., K. R. Atkuri, D. Deb-Basu, A. S. Adler, H. Y. Chang, L. A. Herzenberg and D. W. Felsher (2006). "MYC Can Induce DNA Breaks In vivo and In vitro Independent of Reactive Oxygen Species." Cancer Res 66(13): 6598-6605.Abstract
LAH #511
Sanyal, M., Tung, J. W., Karsunky, H., Zeng, H., Selleri, L., Weissman, I. L., Herzenberg, L. A. and Cleary, M. L. (2007). "B Cell Development Fails in the Absence of the Pbx1 Proto-Oncogene." Blood.Abstract
LAH #512
Tung, J. W., M. D. Mrazek, Y. Yang, L. A. Herzenberg and L. A. Herzenberg (2006). "Phenotypically distinct B cell development pathways map to the three B cell lineages in the mouse." Proc Natl Acad Sci U S A 103(16): 6293-8.Abstract
LAH #513
Tirouvanziam, R., C. K. Conrad, T. Bottiglieri, L. A. Herzenberg, R. B. Moss and L. A. Herzenberg (2006). "High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis." Proc Natl Acad Sci U S A 103(12): 4628-33.Abstract
LAH #514
Weerkamp, F., M. R. Baert, B. A. Naber, E. E. Koster, E. F. de Haas, K. R. Atkuri, J. J. van Dongen, L. A. Herzenberg and F. J. Staal (2006). "Wnt signaling in the thymus is regulated by differential expression of intracellular signaling molecules." Proc Natl Acad Sci U S A 103(9): 3322-6.Abstract
LAH #515
Herzenberg, L. A. and J. W. Tung (2006). "B cell lineages: documented at last!" Nat Immunol 7(3): 225-6.
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LAH #516
LAH #516-1 Supplemental Material
Herzenberg, L. A., J. Tung, W. A. Moore, L. A. Herzenberg and D. R. Parks (2006). "Interpreting flow cytometry data: a guide for the perplexed." Nat Immunol 7(7): 681-5.
LAH #517
Parks, D. R., M. Roederer and W. A. Moore (2006). "A new "Logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data." Cytometry A 69(6): 541-51.Abstract
RESULTS: We identified a set of criteria for creating data visualization methods that accommodate the scaling difficulties presented by flow cytometry data. On the basis of these, we developed a new data visualization method that provides important advantages over linear or logarithmic scaling for display of flow cytometry data, a scaling we refer to as "Logicle" scaling. Logicle functions represent a particular generalization of the hyperbolic sine function with one more adjustable parameter than linear or logarithmic functions. Finally, we developed methods for objectively and automatically selecting an appropriate value for this parameter.
CONCLUSIONS: The Logicle display method provides more complete, appropriate, and readily interpretable representations of data that includes populations with low-to-zero means, including distributions resulting from fluorescence compensation procedures, than can be produced using either logarithmic or linear displays. The method includes a specific algorithm for evaluating actual data distributions and deriving parameters of the Logicle scaling function appropriate for optimal display of that data. It is critical to note that Logicle visualization does not change the data values or the descriptive statistics computed from them.
LAH #518
Fukuhara, T., T. Hosoya, S. Shimizu, K. Sumi, T. Oshiro, Y. Yoshinaka, M. Suzuki, N. Yamamoto, L. A. Herzenberg, L. A. Herzenberg and M. Hagiwara (2006). "Utilization of host SR protein kinases and RNA-splicing machinery during viral replication." Proc Natl Acad Sci U S A.Abstract
LAH #519
Wang, D., Carroll, G. T., Turro, N. J., Koberstein, J. T., Kovac, P., Saksena, R., Adamo, R., Herzenberg, L. A., Herzenberg, L. A. and Steinman, L. (2007). "Photogenerated Glycan Arrays Identify Immunogenic Sugar Moieties of Bacillus Anthracis Exosporium." Proteomics 7(2): 180-4.Abstract
LAH #520
Tung, J. W. and Herzenberg, L. A. (2007). "Unraveling B-1 Progenitors." Curr Opin Immunol.Abstract
LAH #521
Yang, Y., Tung, J. W., Ghosn, E. E., Herzenberg, L. A. and Herzenberg, L. A. (2007). "Division and Differentiation of Natural Antibody-Producing Cells in Mouse Spleen." Proc Natl Acad Sci U S A 104(11): 4542-6.Abstract
LAH #522
Atkuri, K. R., Herzenberg, L. A., Niemi, A. K., Cowan, T. and Herzenberg, L. A. (2007). "Importance of Culturing Primary Lymphocytes at Physiological Oxygen Levels." Proc Natl Acad Sci U S A 104(11): 4547-52.Abstract
LAH #523
Atkuri, K. R., Mantovani, J. J., Herzenberg, L. A. and Herzenberg, L. A. (2007). "N-Acetylcysteine-a Safe Antidote for Cysteine/Glutathione Deficiency." Curr Opin Pharmacol.Abstract
LAH #524
Baumgarth, N., Choi, Y. S., Rothaeusler, K., Yang, Y. and Herzenberg, L. A. (2007). B Cell Lineage Contributions to Antiviral Host Responses. Specialization and Complementation of Humoral Immune Responses to Infection. T. Manser. Berlin Heidelberg, Springer-Verlag. 319: 41-61.Abstract
LAH #526
Tung, J. W., Heydari, K., Tirouvanziam, R., Sahaf, B., Parks, D. R., Herzenberg, L. A. and Herzenberg, L. A. (2007). "Modern Flow Cytometry: A Practical Approach." Clinics in Laboratory Medicine 27(3): 453–468.Abstract
LAH #527
Herzenberg, L. A. (2007). "Epitope-Specific Regulation: The Elephant in the Bathtub." Nat Immunol 8(8): 783-6.
LAH #528
Ghosn, E. E., Y. Yang, J. Tung and L. A. Herzenberg (2008). "CD11b expression distinguishes sequential stages of peritoneal B-1 development." Proc Natl Acad Sci U S A 105(13): 5195-5200.Abstract
LAH #529
LAH #529 Supplemental Material
Tirouvanziam, R., Y. Gernez, et al. (2008). "Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways." Proc Natl Acad Sci U S A 105(11): 4335-9.Abstract
LAH #530
Sahaf, B., K. Atkuri, K. Heydari, M. Malipatlolla, J. Rappaport, E. Regulier, L. A. Herzenberg and L. A. Herzenberg (2008). "Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease." Proc Natl Acad Sci U S A 105(13): 5111-6.Abstract
LAH #531
Herzenberg, L. A., T. Rindfleisch and L. A. Herzenberg (2008). "Joshua Lederberg: the Stanford Years (1958-1978)." Annu Rev Genet 42: 19-25.
LAH #532
LAH #532 Supplemental Material
Tirouvanziam, R., Y. Gernez, C. K. Conrad, R. B. Moss, I. Schrijver, C. E. Dunn, Z. A. Davies and L. A. Herzenberg (2008). "Profound functional and signaling changes in viable inflammatory neutrophils homing to cystic fibrosis airways." Proc Natl Acad Sci U S A 105(11): 4335-4339. Abstract
LAH #533
LAH #533 Supplemental Material
Cole, L. E., Y. Yang, K. L. Elkins, E. T. Fernandez, N. Qureshi, M. J. Shlomchik, L. A. Herzenberg, L. A. Herzenberg and S. N. Vogel (2009). "Antigen-specific B-1a antibodies induced by Francisella tularensis LPS provide long-term protection against F. tularensis LVS challenge." Proceedings of the National Academy of Sciences: Abstract
LAH #534
Walther, G., N. Zimmerman, W. Moore, D. Parks, S. Meehan, I. Belitskaya, J. Pan and L. Herzenberg (2009). "Automatic clustering of flow cytometry data with density-based merging." Adv Bioinformatics: 686759. Abstract
LAH #535
Makam, M., D. Diaz, J. Laval, Y. Gernez, C. K. Conrad, C. E. Dunn, Z. A. Davies, R. B. Moss, L. A. Herzenberg and R. Tirouvanziam (2009). "Activation of critical, host-induced, metabolic and stress pathways marks neutrophil entry into cystic fibrosis lungs." Proc Natl Acad Sci U S A 106(14): 5779-5783. Abstract
LAH #536
LAH #536 Supplemental Information
Atkuri, K. R., T. M. Cowan, T. Kwan, A. Ng, L. A. Herzenberg, L. A. Herzenberg and G. M. Enns (2009). "Inherited disorders affecting mitochondrial function are associated with glutathione deficiency and hypocitrullinemia." Proc Natl Acad Sci U S A. Abstract
LAH #537
LAH $537 Supplemental Material
Ghosn, E. E., A. A. Cassado, G. R. Govoni, T. Fukuhara, Y. Yang, D. M. Monack, K. R. Bortoluci, S. R. Almeida and L. A. Herzenberg (2010). "Two physically, functionally, and developmentally distinct peritoneal macrophage subsets." Proc Natl Acad Sci U S A 107(6): 2568-2573. Abstract
The Fluorescence Activated Cell Separator (FACS) separates cells according to fluorescence, light-scattering characteristics, or selected combinations of these two parameters. The instrument can process up to 5000 cells/sec and separate two nearly pure fractions with independently specified ranges of fluorescence and light- scattering cross sections for each fraction. The FACS can differentiate between viable and dead cells on the basis of light scattering. Appropriate threshold setting permits "gating out" of the dead-cell population so that cell populations can be separated or analyzed on the basis of viable-cell content. Using the FACS in combination with fluorescein-conjugated antigens to visualize antigen-binding cells enriched up to 400-fold from spleens of primed and unprimed mice. We have directly demonstrated that antigen-binding cells in both primed and unprimed mouse spleen contain the precursors of antibody-forming cells. Moreover, the antigen-binding precursor cells are functionally specific, and the avidity of the antibody formed is directly correlated with the avidity of the antigen receptors on the antigen-binding precursor cells.
LAH #538
Song, K., D. L. Bolton, R. L. Wilson, J. V. Camp, S. Bao, J. J. Mattapallil, L. A. Herzenberg, L. A. Herzenberg, C. A. Andrews, J. C. Sadoff, J. Goudsmit, M. G. Pau, R. A. Seder, P. A. Kozlowski, G. J. Nabel, M. Roederer and S. S. Rao (2010). "Genetic immunization in the lung induces potent local and systemic immune responses." Proceedings of the National Academy of Sciences 107(51): 22213-22218.. Abstract